| CatalogCode: | | NB200-533 |
| ProductName: | | Dynamin 1 Antibody |
| Product Description: | | Mouse Monoclonal anti-Dynamin 1 (4E67) |
| Clone: | | 4E67 |
| Clonality: | | Monoclonal |
| Immunogen: | | Recombinant His6-tagged human dynamin (aa1-750). |
| Epitope: | | GNRGMEDLIPLVNRLQD |
| Specificity: | | Recognizes a 100kD protein, corresponding to dynamin. |
| CrossReactivity: | | Cross-reacts with Cow, Chicken, Human, Mouse and Rat. Not yet tested in other species. |
| Packaging: | | 0.05 mg Affinity purified Mouse ascites. |
| Uses: | | IP: Use at an assay dependent dilution. IF: Use at assay dependent dilution. Formaldehyde perfused and fixed rat brain shows synaptic immunoflorescence. Not tested in other applications. Optimal dilutions/concentrations should be determined by the end user. |
| Localization: | | Cytoplasm; cytoskeleton. Microtubule associated. |
| Control: | | Mouse Brain Tissue Extract |
| Background: | | Dynamin is a 100kD GTP binding protein that was first identified as a protein able to bind microtubules (1). A mutation in the Drosophila gene shits, encoding a dynamin homologue, prevents synaptic vesicle recycling and causes temperature-sensitive paralysis, implicating dynamin in the formation of coated vesicles (2). Dynamin may mediate vesicle formation by assembling into a ring structure around the necks of invaginated coated pits that may eventually lead to the closure of the neck and formation of vesicles (3). Amphiphysin, dynamin, clathrin and AP2 form a complex during synaptic vesicle endocytosis, which is regulated by the phosphorylated state of different components in this complex (4). To date, three genes have been found to encode dynamin isoforms. Dynamin 1 is neuronal specific. Dynamin 2 is ubiquitously expressed. Dynamin 3 localizes to testis, brain and lung (5). |
| Storage: | | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze thaw cycles. |
| Purity: | | Affinity purified |
| Isotype: | | IgG |
| Host_Name: | | Mouse |
| Buffer: | | PBS, 0.1mM PMSF, before the addition of glycerol to 40% |
| ListPrice: | | 325 |
| AppSummary: | | IF, IP, WB |
| SpeciesSummary: | | Bv, Ch, Hu, Mu, Rt |
| ALTnames: | | Anti-D100 antibody; Anti-DNM antibody; Anti-EC 3.6.5.5 antibody; Anti-B dynamin antibody; Anti-DNM 1 antibody; Anti-DNM antibody; Anti-DNM1 antibody Dynamin antibody; Anti-Dynamin BREDNM19 antibody; Anti-Dynamin1 antibody |
| ProteinTarget: | | Dynamin 1 |
| PackageSize: | | 0.05 mg |
| more information: | | company product webpage |