| CatalogCode: | NB200-303 |
| ProductName: | Estrogen Receptor Beta Antibody |
| Product Description: | Mouse Monoclonal anti-Estrogen Receptor Beta (6A12) |
| Clone: | 6A12 |
| Clonality: | Monoclonal |
| Immunogen: | Recombinant 6-His fusion protein encoding amino acids 1-153 of human ER-ß expressed in E. coli. |
| Specificity: | This antibody is specific for Estrogen receptor-Beta. |
| CrossReactivity: | NB 200-303 reacts with human ER-Beta |
| Packaging: | 0.1 mg Mouse ascites. |
| Uses: | By Western blot, this antibody recognizes ER-beta. Suggested working dilutions: * Western Blot 1-5 ug/ml *The investigator should determine the optimal working dilution for a specific application. |
| Control: | Recombinant fusion protein. |
| Background: | The human ER-ß is a newly discovered estrogen receptor initially cloned and characterized from testis. The size and structure of ER-ß is very similar to ER-a with the ligand and DNA binding domains being highly conserved, while the amino terminus which serves as their transactivation domains have diverged significantly. Similar in function to ER-a, ER-ß binds to estrogen with a high affinity and regulates estrogen specific gene activation through direct interaction with estrogen response elements (ERE's). |
| ProductRef: | 1. Bianco, N.R., et al. Functional implications of antiestrogen induction of quinone reductase: Inhibition of estrogen-induced deoxyribonucleic acid damage. Mol. Endocrinol. 17:1344-1355, 2003. 2. Fuqua, S.A.W., et al. Estrogen receptor beta prtoein in human breast cancer: correlation with clinical tumor parameters. Cancer Res. 63:2434-2439, 2003. 3. Saji, S., et al. Expression of estrogen receptor (ER) (beta)cx protein in ER(alpha)-positive breast cancer: specific correlation with progesterone receptor. Cancer Res. 62(17):4849-4853, 2002. 4. Speirs, V., et al. Distinct expression patterns of ER alpha and ER beta in normal human mammary gland. J. Clin. Pathol. 55(5): 371-374, 2002. 5. Konstatinopoulosa, P.A., et al. Oestrogen receptor beta (ERb) is abundantly expressed in normal colonic mucosa, but declines in colon adenocarcinoma paralleling the tumor's dedifferentiation. Euro. J. of Cancer 39:1251-1258, 2003. 6. Cooke, F.J., et al. Epifenetic control of the oestrogen receptor genes in breast cancer. Brit. J. of Surgery. 90:42-43, 2003. 7. Tee, M.K., et al. Estradiol and selective estrogen receptor modulators differentially regulate target genes with estrogen receptors alpha and beta. Mol. Biol. Cel.. 15:1262, 2004. 8. Hopp, T.A., et al. Low levels of estrogen recpetor beta protein predict resistance to tamoxifen therapy in breast cancer. Clin. Can. Res. 10:7490-7499, 2004. |
| Storage: | Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles. |
| Isotype: | IgG2b/k |
| Host_Name: | Mouse |
| Buffer: | PBS [pH7.4] contains no preservative |
| ListPrice: | 285 |
| AppSummary: | WB |
| SpeciesSummary: | Hu |
| ALTnames: | anti-ER beta antibody |
| ProteinTarget: | Estrogen Receptor (beta) |
| PackageSize: | 0.1 mg |
| GeneralRef: | 1.Mosselman, S., Polman, J., and Dijkema, R. ER beta: Identification and characterization of a novel human estrogen receptor. FEBS Letter. 392: 49-53, 1996. 2.Dotlaw, H., Leygue, E., Watson, P.H., and L.C. Murphy. Expression of estrogen receptor-beta in human breast tumors. J. Clin. Endo. Metab. 82: 2371-2374, 1996. 3.Kuiper, G.G.J.M., Carlsson, B., Grandien, K., Enmark, E., Haggblad, J., Nilsson, S., and J.A. Gustaffson. Comparison of the ligand binding specificity and transcript tissue distribution of estrogen receptor d and b. Endocrinology 138: 863-870, 1997. 4. Dotzlaw, H., et al. Ecpression of estrogen receptor-beta in human breast tumors. J. Clin. Endo. Metab. 82:2371-2374, 1996. |
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