| CatalogCode: | NB200-149 |
| ProductName: | SOCS2 Antibody |
| Product Description: | Rabbit Polyclonal anti-SOCS2 |
| Clonality: | Polyclonal |
| Immunogen: | Recombinant C-terminal SOCS2 protein fragment, including the SH2 domain and part of the SOCS box. |
| Specificity: | NB 200-149 is specific for SOCS2 protein only. |
| CrossReactivity: | NB 200-149 can be used to detect human and mouse SOCS2 protein. |
| Packaging: | 0.1 mg protein A purified Rabbit antisera. |
| Uses: | A band should be seen at ~18 kD, in Western blotting. Can be used for immunoprecipitation. Suggested working dilutions: * Western Blot 1:1,000 (ECL) Immunohistochemistry ND Immunoprecipitation 1:50 *The investigator should determine the optimal dilution for a specific application. |
| Background: | The eight members of the recently identified Suppressor of Cytokines Signalling (SOCS) family are SOCS1, SOCS2, SOCS3, SOCS4, SOCS5, SOCS6, SOCS7, and CIS. Structurally the SOCS proteins are composed of an N-terminal region of variable length and amino acid composition, a central SH2 domain, and a C-terminal motif called the SOCS box. The SOCS proteins appear to form part of a classical negative feedback loop that regulates cytokine signal transduction. Transcription of each of the SOCS genes occurs rapidly in vitro and in vivo in response to cytokines, and once produced, the various members of the SOCS family appear to inhibit signalling in different ways(1, 2). SOCS2, also known as CIS2 or SSI1, is capable of inhibiting cytokine signaling by interleukin-6 (IL-6) and growth hormone(3). SOCS2 interacts with the phosphorylated insulin-like growth factor (IGF-1) receptor, where it may play a regulatory role in cellular growth, differentiation, and inhibition of apoptosis via the Ras and PI3K signaling pathways. In humans, SOCS2 mRNA is expressed widely in both fetal and adult tissues, including fetal kidney and adult heart, skeletal muscle, pancreas, and liver(4).In rats, SOCS2 mRNA exhibits high levels of expression in liver and heart(5), and in the mouse nervous system, SOCS2 is expressed exclusively by neurons, where it is switched on developmentally in concordance with neuronal differentiation(6). |
| Storage: | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze thaw cycles. |
| Purity: | protein A purified |
| Host_Name: | Rabbit |
| Buffer: | 50% Glycerol, PBS |
| ListPrice: | 290 |
| AppSummary: | IHC, IP, WB |
| SpeciesSummary: | Hu, Mu |
| ProteinTarget: | SOCS 2 |
| PackageSize: | 0.1 mg |
| GeneralRef: | 1.Starr, R. et al. Nature 387(6636):917-921 (1997) 2.O-Shea, J.J. et al. Cell. 109 Suppl:S121-131 (2002) 3.Kreb, D.L. and Hilton, D.J. J. Cell Sci. 113:2813-2819 (2000) 4.Dey, B.R. et al. J. Biol. Chem 273(37):24095-24101 (1998) 5.Tollet-Egnell, P. et al. Endocrinology 140 (8):3963-3974 (1999) 6.Polizzotto, M.N. et al. J. Comp. Neurol. 423(2):348-358 (2000) |
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