| CatalogCode: | | NB120-9478 |
| ProductName: | | Bcl-x Antibody |
| Product Description: | | Mouse Monoclonal anti-Bcl-x (2H12) |
| Clone: | | 2H12 |
| Clonality: | | Monoclonal |
| Immunogen: | | Synthetic peptide: C-QSNRELVVDFLS, corresponding to amino acids 3-14 of Human Bcl x. |
| Specificity: | | This antibody recognizes a 27 kDa protein identified as bcl-X protein. This antibody is highly specific to bcl-X and does not show cross reactivity with bcl-2 alpha or bax protein. |
| CrossReactivity: | | Cross-reacts with Human, Rat, Mouse and Pig.Not yet tested in other species. |
| Packaging: | | 0.05 mg Tissue culture supernatant Mouse ascites. |
| Uses: | | Immunohistochemistry: use at a dilution of 1 / 20 - 1 / 50 for 30 - 60 mins at RT (ABC method). Immunoprecipitation: use at a dilution of 1 / 50.Western Blot: use at a dilution of 1 / 50 - 1 / 100.Not tested in other applications.Optimal dilutions / concentrations should be determined by the end user.By Western blot, this antibody detects a band of 27 kDa, which corresponds to the predicted molecular weight of Bcl-x.Formalin fixed paraffin embedded tissue sections require high temperature antigen unmasking with 10mM citrate buffer pH 6.0 prior to immunostaining. |
| Localization: | | Mitochondrial membranes and perinuclear envelope |
| Control: | | Hodgkin?s lymphoma. |
| Storage: | | Store at 4C. Do not freeze. |
| Purity: | | Tissue culture supernatant |
| Isotype: | | IgG2a |
| Host_Name: | | Mouse |
| Buffer: | | n/a |
| ListPrice: | | 295 |
| AppSummary: | | IHC-Fr, IHC-P, IP, WB |
| SpeciesSummary: | | Hu, Mu, Po, Rt |
| ProteinTarget: | | Bcl-x |
| PackageSize: | | 0.05 mg |
| GeneralRef: | | General / background references:Hsu YT & Youle RJ Nonionic detergents induce dimerization among members of the Bcl-2 family. J Biol Chem 272:13829-34 (1997). Hsu YT et al. Cytosol-to-membrane redistribution of Bax and Bcl-X(L) during apoptosis. Proc Natl Acad Sci U S A 94:3668-72 (1997). Wolter KG et al. Movement of Bax from the cytosol to mitochondria during apoptosis. J Cell Biol 139:1281-92 (1997). |
| more information: | | company product webpage |