| CatalogCode: | NB120-873 |
| ProductName: | Ki-67 Antibody |
| Product Description: | Mouse Monoclonal anti-Ki-67 (MB67) |
| Clone: | MB67 |
| Clonality: | Monoclonal |
| Immunogen: | Human recombinant Ki67 proteincontaining the C-terminal 340 amino acids. |
| Specificity: | This antibody is specific to the Ki-67 nuclear antigen of 345 and 395 kD. It recognizes the native as well as recombinant fragments of Ki-67. Ki-67 nuclear antigen is associated with cell proliferation and is found throughout the cell cycle |
| CrossReactivity: | Cross-reacts with Human.Not yet tested in other species. |
| Packaging: | 6 ml Purified Mouse IgG. |
| Uses: | This antibody has been pretitered and quality controlled to work on formalin-fixed paraffin-embedded as well as acetone fixed cryostat tissue sections. No further titration is required. We suggest an incubation period of 60 minutes at room temperature. Paraffin-embedded tissue sections require high temperature unmasking |
| Localization: | Nuclear |
| Control: | Tonsil or Breast carcinoma. |
| Background: | Ki67 antigen is the prototypic cell cycle related nuclear protein, expressed by proliferating cells in all phases of the active cell cycle (G1, S, G2 and M phase). It is absent in resting (G0) cells. Ki67 antibodies are useful in establishing the cell growing fraction in neoplasms (immunohistochemically quantified by determining the number of Ki67 positive cells among the total number of resting cells = Ki67 index). In neoplastic tissues the prognostic value is comparable to the tritiated thymidine labelling index. The correlation between low Ki67 index and histologically low grade tumours is strong. Ki67 is routinely used as a neuronal marker of cell cycling and proliferation. |
| Storage: | Store at 4C. Do not freeze. |
| Isotype: | IgG1 kappa |
| Host_Name: | Mouse |
| Buffer: | PBS with sodium azide |
| ListPrice: | 285 |
| AppSummary: | IHC-Fr, IHC-P |
| SpeciesSummary: | Hu |
| ALTnames: | anti-Antigen identified by monoclonal antibody Ki 67 antibody, anti-Antigen KI67 antibody, anti-KIA antibody, anti-MKI67 antibody, anti-Proliferation related Ki 67 antigen antibody |
| ProteinTarget: | Ki-67 |
| PackageSize: | 6 ml |
| GeneralRef: | General / background references:Key G et al. New Ki-67-equivalent murine monoclonal antibodies (MIB 1-3) generated against bacterially expressed parts of the Ki-67 cDNA containing three 62 base pair repetitive elements encoding for the Ki-67 |