| CatalogCode: | NB120-8420 |
| ProductName: | FLASH Antibody |
| Product Description: | Rabbit Polyclonal anti-FLASH |
| Clonality: | Polyclonal |
| Immunogen: | Peptide corresponding to amino acids 1253 to1268 of human origin, which differ from those of mouseby one amino acid (1,2). |
| CrossReactivity: | Cross-reacts with Human.Expected to cross-react with Mouse due to sequence homology.Not yet tested in other species. |
| Packaging: | 0.05 mg IgG purified Rabbit antisera. |
| Uses: | Western blot at 0.25 to 0.5 ug/ml. |
| Background: | A novel mammalian CED-4 homologous was recently identified and cloned in mouse (1) and human (2) and designated FLASH (for FLICE-associated huge protein). FLASH is involved in Fas induced apoptosis. It is recruited to Fas after the receptor cross-linking. Overexpression of wild type of FLASH facilitates and its dominant negative form inhibits Fas induced apoptosis. FLASH interacts with the DEDs of caspase-8 and FADD through the DED-like domain of FLASH and mediates activation of caspase-8 (1). There are parallels between FLASH and Apaf-1/CED-4 although there are arguments against their structural similarity (1,2). |
| Storage: | Store at 4C. Do not freeze. |
| Purity: | IgG purified |
| Isotype: | IgG |
| Host_Name: | Rabbit |
| Buffer: | PBS with 0.02% sodium azide |
| ListPrice: | 295 |
| AppSummary: | WB |
| SpeciesSummary: | Hu |
| ProteinTarget: | FLASH |
| PackageSize: | 0.05 mg |
| GeneralRef: | General / background references:Imai Y et al. The CED-4-homologous protein FLASH is involved in Fas-mediated activation of caspase-8 during apoptosis. Nature 398:777-85 (1999). Koonin EV et al. Apoptosis. Searching for FLASH domains. Nature 401:662; disc 662-3 (1999). Medema JP Apoptosis. Life and death in a FLASH. Nature 398:756-7 (1999). |