| CatalogCode: | | NB120-8400 |
| ProductName: | | CAD Antibody |
| Product Description: | | Rabbit Polyclonal anti-CAD |
| Clonality: | | Polyclonal |
| Immunogen: | | Synthetic peptide, corresponding to amino acids 314-329 of Mouse CAD. |
| CrossReactivity: | | Cross-reacts with Mouse.Not yet tested in other species. |
| Packaging: | | 0.05 mg IgG purified Rabbit antisera. |
| Uses: | | Western blot at 1:500 to 1:1000 dilution. |
| Localization: | | Cytoplasmic and Nuclear. |
| Control: | | Tissue lysate from murine lung or kidney.A 40 kDa band should be detected. |
| Background: | | Apoptosis is related to many diseases and induced by a family of cell death receptors and their ligands. Cell death signals are transduced by death domain containing adapter molecules and members of the caspase family of proteases. These death signals finally cause the degradation ofchromosomal DNA by activated DNase. A mouse DNase that causes DNA fragmentation was identified recently and designated CAD, which stands for caspase activated deoxyribonuclease (1,2). The human homologue of mouse CAD was more recently identified by two groups independently and termed CPAN and DFF40 (3,4). Human DFF45 and its mouse homologue ICAD are the inhibitors of CPAN/DFF40 and CAD, respectively (1, 2, 5). Upon cleavage of DFF45/ICAD by activated caspase, DFF40/CAD is released and activated and eventually causes the degradation of DNA in the nuclei (1-4). Activation of CAD/DFF40, which causes DNA degradation, is the hallmark of apoptotic cell death. |
| Storage: | | Store at 4C. Do not freeze. |
| Purity: | | IgG purified |
| Isotype: | | IgG |
| Host_Name: | | Rabbit |
| Buffer: | | PBS with 0.02% sodium azide |
| ListPrice: | | 295 |
| AppSummary: | | WB |
| SpeciesSummary: | | Mu |
| ALTnames: | | anti-Caspase activated deoxyribonuclease antibody, anti-Caspase activated DNase antibody, anti-Caspase activated nuclease antibody, anti-CPAN antibody, anti-DFF2 antibody, anti-DFF40 antibody, anti-DFFB antibody, anti-DNA fragmentation factor 40 kD beta subunit antibody, DNA fragmentation factor 40kDa beta polypeptide antibody |
| ProteinTarget: | | CAD |
| PackageSize: | | 0.05 mg |
| GeneralRef: | | General / background references:Halenbeck R et al. CPAN, a human nuclease regulated by the caspase-sensitive inhibitor DFF45. Curr Biol 8:537-40 (1998). Liu X et al. The 40-kDa subunit of DNA fragmentation factor induces DNA fragmentation and chromatin condensation during apoptosis. Proc Natl Acad Sci U S A 95:8461-6 (1998). Sakahira H et al. Cleavage of CAD inhibitor in CAD activation and DNA degradation during apoptosis. Nature 391:96-9 (1998). Enari M et al. A caspase-activated DNase that degrades DNA during apoptosis, and its inhibitor ICAD. Nature 391:43-50 (1998). Liu X et al. DFF, a heterodimeric protein that functions downstream of caspase-3 to trigger DNA fragmentation during apoptosis. Cell 89:175-84 (1997). |
| more information: | | company product webpage |