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| Catalog Code: | NB120-837 |
| Product Name: | Ubiquitin Antibody |
| Product Description: | Rabbit Polyclonal anti-Ubiquitin |
| Clonality: | Polyclonal |
| ListPrice: | 295 |
| Immunogen: | Ubiquitin isolated from cow erythrocytes and conjugated to allchicken gammaglobulins. |
| Specificity: | This antibody is specific for ubiquitin. It also stains with the periphery of senile plaques and neurofibrillay tangles in Alzheimer's disease. |
| Cross Reactivity: | Cross-reacts with Human, Rat, Mouse and Cow. Not yet tested in other species. |
| Packaging: | 0.5 ml Whole Rabbit antisera. |
| Uses: | IHC: This antibody may be diluted to a titer of 1:50-1:100 in an ABC method. Staining Protocol: We suggest an incubation period of 30-60 minutes at room temperature. |
| Localization: | Cytoplasmic and Nuclear |
| Background: | Ubiquitin is a highly conserved protein of about 8.5 kDa molecular weight, which has an ATP dependent role in the targeting of proteins for proteolytic degradation. To perform this function, the protein to be degraded is first covalently attached to the C terminus of ubiquitin, and the ubiquitinated complex is then recognized by a complex of degradative enzymes. Interestingly, ubiquitin also becomes covalently bonded to many types of pathological inclusions, which appear to be resistant to normal degradation. Therefore, ubiquitin antibodies are very useful for studies of these inclusions. For example, the neurofibrillary tangles and paired helical filaments diagnostic of Alzheimer's disease, Lewy bodies seen in Parkinson's disease, and Pick bodies found in Pick's disease are all heavily ubiquitinated and can be readily visualized with ubiquitin antibodies. |
| Storage: | Store at 4C. Do not freeze. |
| Purity: | Whole antisera |
| Isotype: | IgG |
| Host Name: | Rabbit |
| Application Summary: | immunohistochemistry-Fr, immunohistochemistry-P |
| Species Summary: | Bv, Hu, Mu, Rt, |
| Alternate Names: | anti-60S ribosomal protein L40 antibody, anti-HUBCEP52 antibody, anti-RPL40 antibody, anti-Ubiquitin 52 amino acid fusion protein antibody, anti-Ubiquitin A52 residue ribosomal protein fusion product 1 antibody, anti-Ubiquitin carboxyl extension protein 52 antibody, anti-Ubiquitin CEP52 antibody |
| Package Size: | 0.5 ml |
| General References: | General / background references:Mayer RJ et al. Ubiquitin and dementia. Nature 340:193 (1989). Lennox G et al. Ubiquitin is a component of neurofibrillary tangles in a variety of neurodegenerative diseases. Neurosci Lett 94:211-7 (1988). Perry G et al. Ubiquitin is detected in neurofibrillary tangles and senile plaque neurites of Alzheimer disease brains. Proc Natl Acad Sci U S A 84:3033-6 (1987). |
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