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| Catalog Code: | NB120-79883 |
| Product Name: | Dnmt3a Antibody |
| Product Description: | Mouse Monoclonal anti-Dnmt3a (64B1446) |
| Clone: | 64B1446 |
| Clonality: | Monoclonal |
| ListPrice: | 325 |
| Concentration: | 0.5 mg/ml |
| Immunogen: | This antibody was raised against bacteria expressed HIS-tag recombinant mouse Dnmt3a. The epitope was found to lie near the C-terminus (a.a. 705-908) (product citation 5). |
| Epitope: | Found to lie near the C terminus (a.a. 705-908). |
| Cross Reactivity: | Cross-reacts with Human (weakly) and Mouse. Not yet tested in other species. |
| Packaging: | 0.1 mg protein G purified Mouse ascites. |
| Uses: | WB: Detects a band of approximately 120 kDa (predicted molecular weight: 102 kDa). |
| Localization: | Nuclear |
| Background: | Methylation of DNA at cytosine residues plays an important role in regulation of gene expression, genomic imprinting and is essential for mammalian development. Hypermethylation of CpG islands in tumor suppressor genes or hypomethylation of bulk genomic DNA may be linked with development of cancer. To date, 3 families of mammalian DNA methyltransferase genes have been identified which include Dnmt1, Dnmt2 and Dnmt3. Dnmt1 is constitutively expressed in proliferating cells and inactivation of this gene causes global demethylation of genomic DNA and embryonic lethality. Dnmt2 is expressed at low levels in adult tissues and its inactivation does not affect DNA methylation or maintenance of methylation. The Dnmt3 family members, Dnmt3a and Dnmt3b, are strongly expressed in ES cells but their expression is down regulated in differentiating ES cells and is low in adult somatic tissue. Recently, it has been shown that naturally occurring mutations of Dnmt3b gene occurs in patients with a rare autosomal recessive disorder, termed ICF (immunodeficiency, centromeric instability, and facial anomalies) syndrome. |
| Storage: | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze thaw cycles. |
| Purity: | protein G purified |
| Isotype: | IgG1 |
| Host Name: | Mouse |
| Buffer: | PBS containing 0.2% gelatin and 0.05% sodium azide. Sodium azide is highly toxic. |
| Application Summary: | chromatin immunoprecipitation , immunohistochemistry , immunofluorescence , Western Blot 2 ug/ml, Immunocytochemistry , |
| Species Summary: | Hu, Mu, |
| Alternate Names: | anti-DNMT3A2 antibody, anti-M.HsaIIIA antibody |
| Package Size: | 0.1 mg |
| Preservative: | 0.02% Sodium Azide |
| General References: | This product has been used in:Chen T et al. A novel Dnmt3a isoform produced from an alternative promoter localizes to euchromatin and its expression correlates with active de novo methylation. J Biol Chem 277:38746-54 (2002). Dennis K et al. Lsh, a member of the SNF2 family, is required for genome-wide methylation. Genes Dev 15:2940-4 (2001). General / background references:Xie S et al. Cloning, expression and chromosome locations of the human DNMT3 gene family. Gene 236:87-95 (1999). Okano M et al. DNA methyltransferases Dnmt3a and Dnmt3b are essential for de novo methylation and mammalian development. Cell 99:247-57 (1999). Reik W et al. Dissecting de novo methylation. Nat Genet 23:380-2 (1999). Hansen RS et al. The DNMT3B DNA methyltransferase gene is mutated in the ICF immunodeficiency syndrome. Proc Natl Acad Sci U S A 96:14412-7 (1999). |
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