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| Catalog Code: | NB120-7980 |
| Product Name: | Caspase 3 Antibody |
| Product Description: | Rabbit Polyclonal anti-Caspase 3 |
| Clonality: | Polyclonal |
| ListPrice: | 295 |
| Immunogen: | Synthetic peptide (Human) (N terminal). |
| Specificity: | Reacts with caspase-3 p20 subunit and precursor (also designated CPP32). |
| Cross Reactivity: | Cross-reacts with Human. Not yet tested in other species. |
| Packaging: | 1 ml IgG purified Rabbit antisera. |
| Background: | The caspase family of cysteine proteases play a key role in apoptosis. Caspase 3 (also known as CPP32, YAMA and apopain) is the most extensively studied apoptotic protein among caspase family members. Caspase 3 is synthesized as inactive pro enzyme that is processed in cells undergoing apoptosis by self proteolysis and/or cleavage by other upstream proteases (e.g. Caspases 8, 9 and 10). The processed form of Caspase 3 consists of large (17kD) and small (12kD) subunits which associate to form an active enzyme. Caspase 3 is cleaved at Asp28 - Ser29 and Asp175 - Ser176. The active Caspase 3 proteolytically cleaves and activates other caspases (e.g. Caspases 6, 7 and 9), as well as relevant targets in the cells (e.g. PARP and DFF). Alternative splicing of this gene results in two transcript variants which encode the same protein. In immunohistochemical studies Caspase 3 expression has been shown to be widespread but not present in all cell types (e.g. commonly reported in epithelial cells of skin, renal proximal tubules and collecting ducts). Differences in the level of Caspase 3 have been reported in cells of short lived nature (eg germinal centre B cells) and those that are long lived (eg mantle zone B cells). Caspase 3 is the predominant caspase involved in the cleavage of amyloid beta 4A precursor protein, which is associated with neuronal death in Alzheimer's disease |
| Storage: | Store at 4C. Do not freeze. |
| Purity: | IgG purified |
| Isotype: | IgG |
| Host Name: | Rabbit |
| Buffer: | PBS |
| Application Summary: | immunohistochemistry , immunoprecipitation , Western Blot 1/200 |
| Species Summary: | Hu, |
| Alternate Names: | anti-Apopain precursor antibody, anti-CASP3 antibody, anti-Caspase 3 apoptosis related cysteine protease antibody, anti-Caspase 3 p12 subunit antibody, anti-Caspase 3 p17 subunit antibody, anti-CPP 32 antibody, anti-CPP32B antibody, anti-Cysteine protease CPP32 antibody, anti-Human cysteine protease CPP32 isoform alpha mRNA complete cds antibody, anti-ICE3 antibody, anti-LICE antibody, anti-PARP cleavage protease antibody, anti-SCA1 antibody, anti-SREBP cleavage activity 1 antibody, anti-Yama protein antibody |
| Package Size: | 1 ml |
| Preservative: | 0.1% sodium azide |
| General References: | <b>General / background references:</b>Zhang W <i>et al.</i> Involvement of caspases in 4-hydroxy-alkenal-induced apoptosis in human leukemic cells. <i>Free Radic Biol Med</i> <b>30</b>:699-706 (2001). <PUBMED:11295368>Slee EA <i>et al.</i> Executioner caspase-3, -6, and -7 perform distinct, non-redundant roles during the demolition phase of apoptosis. <i>J Biol Chem</i> <b>276</b>:7320-6 (2001). <PUBMED:11058599>Eldadah BA & Faden AI Caspase pathways, neuronal apoptosis, and CNS injury. <i>J Neurotrauma</i> <b>17</b>:811-29 (2000). <PUBMED:11063050>Kruidering M & Evan GI Caspase-8 in apoptosis: the beginning of "the end"? <i>IUBMB Life</i> <b>50</b>:85-90 (2000). <PUBMED:11185963>Salvesen GS & Dixit VM Caspase activation: the induced-proximity model. <i>Proc Natl Acad Sci U S A</i> <b>96</b>:10964-7 (1999). <PUBMED:10500109>Wang L <i>et al.</i> Ich-1, an Ice/ced-3-related gene, encodes both positive and negative regulators of programmed cell death. <i>Cell</i> <b>78</b>:739-50 (1994). <PUBMED:8087842>Wilson KP <i>et al.</i> Structure and mechanism of interleukin-1 beta converting enzyme. <i>Nature</i> <b>370</b>:270-5 (1994). <PUBMED:8035875>Kostura MJ <i>et al.</i> Identification of a monocyte specific pre-interleukin 1 beta convertase activity. <i>Proc Natl Acad Sci U S A</i> <b>86</b>:5227-31 (1989). <PUBMED:2787508> |
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