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| Catalog Code: | NB120-7979 |
| Product Name: | Caspase 2L Antibody |
| Product Description: | Rabbit Polyclonal anti-Caspase 2L |
| Clonality: | Polyclonal |
| ListPrice: | 295 |
| Concentration: | 0.2 mg/ml |
| Immunogen: | Synthetic peptide correspdoning to the C-terminal of human caspase 2L |
| Specificity: | Reacts with caspase-2L p12 subunit. |
| Cross Reactivity: | Cross-reacts with Human, Rat and Mouse. Not yet tested in other species. |
| Packaging: | 1 ml IgG purified Rabbit antisera. |
| Background: | Caspases is a family of mammalian proteases that regulate many of the morphological and biochemical features of apoptosis. The currently identified caspases can be divided into three groups: apoptotic initiators, apoptotic executioners, and inflammatory mediators (1,2). Caspase-2, 8, and 9 have been identified as apoptotic initiators (3,4). Caspase-2 (also designated Nedd-2/ICH-1) can be alternatively spliced to produce two distinct Caspase-2 mRNA species. One mRNA species encodes a protein of 435 amino acids in length and the mRNA splice variant encodes a 312 amino acid protein. The larger protein, Caspase-2L, in an over expressed state, leads to programmed cell death. Conversely the over expression of Caspase-2S protein, a truncated version of Caspase-2L, has been shown to suppress cell death induced by serum deprivation (5). The executioner caspases include caspase-3, 6 and 7. Of these three proteases Caspase-3 is considered to be the essential for the chromatin margination, DNA fragmentation, and nuclear collapse during apoptosis (6). Caspase-1 (also known as ICE) is considered to be a mediator of the inflammatory response by converting the inactive precursor of interleukin-1â to the 17 kDa proinflammatory cytokine, IL-1â (7,8). |
| Storage: | Store at 4C. Do not freeze. |
| Purity: | IgG purified |
| Isotype: | IgG |
| Host Name: | Rabbit |
| Buffer: | PBS, 0.2% gelatin |
| Application Summary: | immunohistochemistry Use at an assay dependent dilution, immunoprecipitation Use at an assay dependent dilution, Western Blot 1/200 |
| Species Summary: | Hu, Mu, Rt, |
| Alternate Names: | anti-Ich-1L antibody |
| Package Size: | 1 ml |
| Preservative: | 0.1% sodium azide |
| Notes Main: | Caspases are mammalian proteases that regulate many of the morphological and biochemical features of apoptosis. The currently identified caspases can be divided into three groups: apoptotic initiators, apoptotic executioners, and inflammatory mediators. |
| General References: | 1. Edadah BA, Faden AI. 2000. Caspase pathways, neuronal apoptosis, and CNS injury. Jounal of Neurotauma 17(10): 811-829 2. Salvesen GS, Dixit VM. 1999. Caspase activation: the induced-proximity model. Proc Natl Acad Sci USA 96(20): 10964-10967 3. Zhang W, He Q, Chan LL, Zhou F, El Naghy M, Thompson EB, Ansari NH. 2001. Involvment of caspases in 4-hydroxy-alkenal-induced apoptosis in human leukemic cells. Free Radic Biol Med 30(6): 699-706 4. Kruidering M, Evan GI. 2000. Caspase-8 in apoptosis: the beginning of the end ? IUBMB 50(2): 85-90 5. Wang L, Miura M, Bergeron L, Zhu H, Yuan J. 1994. Ich-1, an Ice/ced-3-related gene, encodes both positive and negative regulators of programmed cell death. Cell 78(5): 739-750 6. Slee EA, Adrain C, Martin SJ. 2001. Executioner caspase-3, -6, and -7 perform distinct, non-redundant roles during the demolition phase of apoptosis. J Biol Chem 276(10): 7320-7326 7. Kostura MJ, Tocci MJ, Limjuco G, Chin J, Cameron P, Hillman AG, Chartrain NA, Schmidt JA. 1989. Identification of a monocyte specific pre-interleukin 1â convertase activity. Proc Natl Acad Sci USA 86(14): 5227-5231 8. Wilson KP, Black JA, Thompson JA, Kim EE, Griffith JP, Navia MA, Murcko MA, Chambers SP, Aldape RA, Raybuck SA, Livingston DJ. 1994. Structure and mechanism of interleukin-1â converting enzyme. Nature 370(6487): 251-252 |
| Product References: | 1.) Shin S, Lee Y, Kim W, Ko H, Choi H, Kim K. 2005. Caspase-2 primes cancer cells for TRAIL-mediated apoptosis by processing procaspase-8. EMBO J. Oct 19;24(20):3532-42. |
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