| CatalogCode: | NB120-7975 |
| ProductName: | Bad Antibody |
| Product Description: | Rabbit Polyclonal anti-Bad |
| Clonality: | Polyclonal |
| Immunogen: | Synthetic peptide (Mouse) (N terminal). |
| Specificity: | Mouse and rat Bad |
| CrossReactivity: | Cross-reacts with Rat and Mouse.Not yet tested in other species. |
| Packaging: | 1 ml IgG purified Rabbit IgG. |
| Uses: | Western blotting and immunohistochemistry |
| Localization: | Mitochondrial |
| Control: | Serum starved NIH/3T3 |
| Background: | Bcl-2 family of proteins is a key regulator of apoptosis that function to either inhibit or promote cell death. The over expression of members such as Bcl-2 and Bcl-xL inhibit the apoptotic process (1,2). The Bcl-2 family members are also characterized by dimerizing to further modulate apoptosis. Bag-1, for example, has been found to form a heterodimer with Bcl-2 resulting in the enhancement of the anti-apoptotic effect of Bcl-2 (3,4). Other anti-apoptotic Bcl-2 family members include A1, Bcl-xg, Bcl-xb, Mcl-1, BAR, BI-1 and Bcl-w (5). The pro-apoptotic family members include Bax, Bcl-xS, Bad, Bak, NBK, BID, Hrk, Bok, Bim, Noxa and Diva. Bax and Bak have been shown to play a critical role in cytochrome c release from mitochondria and thus initiate apoptosis (6). Bad plays a critical role in the Bax-mediated apoptosis pathway by dimerizing with Bcl-xL, causing the displacment of Bax. The displacement of Bax allows apoptosis to proceed (7). Bcl-xS, a shorter version of Bcl-xL (lacking amino acids 126-188), apparently utilizes a different pathway than Bax to induce cell death. Some research suggests that Bcl-xS uses a novel mechanism for regulating caspase or it may use an alternate cell death effector pathway (8,9). |
| ProductRef: | C Clybouw, B E L Mchichi, A Hadji, A Portier, M T Auffredou, D Arnoult, G Leca and A Vazquez. 2008. TGFbeta-mediated apoptosis of Burkitt's lymphoma BL41 cells is associated with the relocation of mitochondrial BimEL. Oncogene advance online publication 14 January 2008. Western blotting and immunohistochemistry |
| Storage: | Store at 4C. Do not freeze. |
| Purity: | IgG purified |
| Isotype: | IgG |
| Host_Name: | Rabbit |
| Buffer: | PBS with 0.1% sodium azide |
| ListPrice: | 285 |
| AppSummary: | IHC-Fr, WB |
| SpeciesSummary: | Mu, Rt |
| ALTnames: | anti-Bcl-2 binding component 6 antibody, anti-BBC 2 antibody, anti-BBC2 antibody, anti-BBC6 antibody, anti-Bcl 2 Antagonist of Cell Death antibody, anti-Bcl 2 Binding Component 6 antibody, anti-BCL X / BCL 2 Binding Protein antibody, anti-BCL X Binding Protein antibody, anti-Bcl XL/Bcl 2 Associated Death Promoter antibody, anti-Bcl2 Associated Death Promoter antibody, anti-Bcl2 Like 8 Protein antibody, anti-BCL2L8 antibody, anti-BclXL antibody, anti-Proapoptotic BH3 Only Protein antibody |
| ProteinTarget: | Bad |
| PackageSize: | 1 ml |
| GeneralRef: | General / background references:Coldwell MJ et al. The p36 isoform of BAG-1 is translated by internal ribosome entry following heat shock. Oncogene 20:4095-100 (2001). Bae J et al. Underphosphorylated BAD interacts with diverse antiapoptotic Bcl-2 family proteins to regulate apoptosis. Apoptosis 6:319-30 (2001). Wei MC et al. Proapoptotic BAX and BAK: a requisite gateway to mitochondrial dysfunction and death. Science 292:727-30 (2001). Fridman JS et al. Cytochrome c depletion upon expression of Bcl-XS. J Biol Chem 276:4205-10 (2001). Lindenboim L et al. Bcl-xS and Bax induce different apoptotic pathways in PC12 cells. Oncogene 19:1783-93 (2000). Huang Z Bcl-2 family proteins as targets for anticancer drug design. Oncogene 19:6627-31 (2000). Eversole-Cire P et al. Synergistic effect of Bcl-2 and BAG-1 on the prevention of photoreceptor cell death. Invest Ophthalmol Vis Sci 41:1953-61 (2000). Reed JC Double identity for proteins of the Bcl-2 family. Nature 387:773-6 (1997). Yang E et al. Bad, a heterodimeric partner for Bcl-XL and Bcl-2, displaces Bax and promotes cell death. Cell 80:285-91 (1995). |
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