| CatalogCode: | NB120-7972 |
| ProductName: | NF-kB Antibody |
| Product Description: | Rabbit Polyclonal anti-NF-kB |
| Clonality: | Polyclonal |
| Immunogen: | Synthetic peptide (Mouse) (C terminal). |
| Specificity: | Reacts with NFkB p52 and p100 |
| CrossReactivity: | Cross-reacts with Human, Rat and Mouse. Not yet tested in other species. |
| Packaging: | 1 ml IgG purified Rabbit antisera. |
| Control: | Jurkat |
| Background: | NFkB is formed through the association of multiple subunits, either as a homodimer or heterodimer. Subunits have been identified as p50 (NFkB1), p65 (RelA), c-Rel, RelB and p52 (NFkB2). The classic NFkB form exists as a p50-p65 heterodimer and predominates in many cell types. Many of the possible combinatorial forms of homo- and heterodimers have been identified and growing evidence indicates that different forms of NFkB have different functions in cells. Interestingly, both the p50 and p52 subunits are derived from the precursor proteins p105 and p100 respectively, that each contain multiple copies of the so called ankyrin repeat at their C termini. Nuclear translocation of NFkB is confirmed by the use of electrophorectic mobility shift assays or by immunoblotting with nuclear extracts. The subunit composition of NFkB is confirmed by the use of antibodies that "supershift" the DNA/protein complex. |
| Storage: | Store at 4C. Do not freeze. |
| Purity: | IgG purified |
| Isotype: | IgG |
| Host_Name: | Rabbit |
| Buffer: | PBS |
| ListPrice: | 295 |
| AppSummary: | IP, WB, IHC-Fr |
| SpeciesSummary: | Hu, Mu, Rt |
| ALTnames: | anti-NFKB 3 antibody, anti-NFKB3 antibody, anti-Nuclear Factor NF Kappa B p65 Subunit antibody, anti-Nuclear Factor Of Kappa Light Polypeptide Gene Enhancer In B Cells antibody, anti-RELA antibody, anti-Transcription Factor p65 antibody, anti-V Rel Avian Reticuloendotheliosis Viral Oncogene Homolog A antibody |
| ProteinTarget: | NF-kB (p52) |
| PackageSize: | 1 ml |
| GeneralRef: | General / background references:Valen G et al. Nuclear factor kappa-B and the heart. J Am Coll Cardiol 38:307-14 (2001). Hatada EN et al. NF-kappaB and the innate immune response. Curr Opin Immunol 12:52-8 (2000). Lentsch AB & Ward PA The NFkappaBb/IkappaB system in acute inflammation. Arch Immunol Ther Exp (Warsz) 48:59-63 (2000). Magnani M et al. The ubiquitin-dependent proteolytic system and other potential targets for the modulation of nuclear factor-kB (NF-kB). Curr Drug Targets 1:387-99 (2000). Sengchanthalangsy LL et al. Characterization of the dimer interface of transcription factor NFkappaB p50 homodimer. J Mol Biol 289:1029-40 (1999). Ho E & Bray TM Antioxidants, NFkappaB activation, and diabetogenesis. Proc Soc Exp Biol Med 222:205-13 (1999). Chen FE et al. Construction, expression, purification and functional analysis of recombinant NFkappaB p50/p65 heterodimer. Protein Eng 12:423-8 (1999). Huang DB et al. The role of DNA in the mechanism of NFkappaB dimer formation: crystal structures of the dimerization domains of the p50 and p65 subunits. Structure 5:1427-36 (1997). |
| NotesMain: | Members of the rel/NFkappaB family of transcription factors are involved in the regulation of cellular responses, such as growth, development, and the inflammatory response. They share a structural motif known as the rel homology region (RHR), the C-terminal one third of which mediates protein dimerization. Complexes of p50 (NF-kB1) or p52 (NF-kB2) are generated through the processing of p105 and p100 precursors, respectively. These are usually associated with members of the Rel family (p65, c-Rel, Rel B). The homo- and heterodimer formed through combinations of NF-kB/Rel proteins bind distinct kB sites to regulate the transcription of different genes. |
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