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| Catalog Code: | NB120-7968 |
| Product Name: | STAT5a Antibody |
| Product Description: | Rabbit Polyclonal anti-STAT5a |
| Clonality: | Polyclonal |
| ListPrice: | 295 |
| Concentration: | 200 µg/ml mg/m |
| Immunogen: | Synthetic peptide (Mouse) (C terminal). |
| Specificity: | Reacts with STAT5a. It does not cross-react with STAT5b. |
| Cross Reactivity: | Cross-reacts with Human, Rat and Mouse.Not yet tested in other species. |
| Packaging: | 1 ml IgG purified Rabbit IgG. |
| Uses: | Western blotting, IP, and IHC. It does not cross-react with Stat5b. Western blotting starting dilution: 1:200. |
| Localization: | Nuclear |
| Background: | Signal Transducers and Activators of Transcription (STATs) are a family of cytoplasmic proteins that participate in cellular responses to cytokines and growth factors (1). Many cytokines involved in immune responses utilize the Jak-STAT signaling pathway. Jaks are receptor-associated protein tyrosine kinases, and STATs are activated by tyrosine phosphorylation (2). Abnormal signaling of the JAK-STAT pathway has been implicated in hematopoietic disorders including severe combined immunodeficiency and leukemia (4). STATs have been implicated in programming gene expression in biological events such as embryonic development, programmed cell death, organogenesis, innate immunity, adaptive immunity and cell growth regulation in many organisms (3). STAT1 knockout mice are defective in interferon-mediated functions. STAT4 and STAT6 knockout mice show defective responses to IL-12 and IL-4, respectively. Analyses of STAT5a and STAT5b knockout mice reveal important roles in prolactin-mediated mammary gland development and growth hormone-mediated induction of sexual dimorphism, respectively. Conditional knockout study of STAT3 demonstrates its critical roles in cytokine-mediated functions in several tissues, including T cells, macrophages, skin, and mammary gland (5). Abnormal activity of certain STAT family members, particularly STAT3 and STAT5, is associated with a wide variety of human malignancies, including hematologic, breast, head and neck, and prostate cancers (1). STAT5A and STAT6, are selectively activated when the heart is subjected to ischemic injury, whereas activation of STAT3 and STAT5A is involved in myocardial hypertrophy (6). STAT5 and STAT3, as these two STAT molecules are required for normal breast development and involution, respectively, and may play an important role in breast carcinogenesis (7). The activation of STAT5 proteins (STAT5a and STAT5b) is one of the earliest signaling events mediated by IL-2 family cytokines (8). |
| Storage: | Store at 4C. Do not freeze. |
| Purity: | IgG purified |
| Isotype: | IgG |
| Host Name: | Rabbit |
| Buffer: | PBS with 0.1% sodium azide |
| Application Summary: | immunohistochemistry Fr, immunoprecipitation , Western Blot 1:200 |
| Species Summary: | Hu, Mu, Rt |
| Alternate Names: | anti-Mammary gland factor antibody, anti-MGF antibody, anti-Signal transducer and activator of transcription 5A antibody, anti-STAT 5 antibody, anti-STAT 5A antibody, anti-STAT5 antibody |
| Package Size: | 1 ml |
| Preservative: | 0.1 % sodium azide, 0.2% gelatin |
| Notes Main: | STATs are Signal Transducers and Activators of Transcription STATs are a family of cytoplasmic proteins that participate in cellular responses to cytokines and growth factors. Many cytokines involved in immune responses utilize the Jak-STAT signaling pathway. Jaks are receptor-associated protein tyrosine kinases, and STATs are activated by tyrosine phosphorylation. Abnormal signaling of the JAK-STAT pathway has been implicated in hematopoietic disorders including SCID and leukemia, and programming gene expression in biological events such as embryonic development, apoptosis, organogenesis, innate immunity, adaptive immunity and cell growth regulation. STAT1 knockout mice are defective in interferon-mediated functions. |
| General References: | General / background references:Mascareno E & Siddiqui MA The role of Jak/STAT signaling in heart tissue renin-angiotensin system. Mol Cell Biochem 212:171-5 (2000). Bromberg J Signal transducers and activators of transcription as regulators of growth, apoptosis and breast development. Breast Cancer Res 2:86-90 (2000). Takeda K & Akira S STAT family of transcription factors in cytokine-mediated biological responses. Cytokine Growth Factor Rev 11:199-207 (2000). Nosaka T & Kitamura T Janus kinases (JAKs) and signal transducers and activators of transcription (STATs) in hematopoietic cells. Int J Hematol 71:309-19 (2000). Turkson J & Jove R STAT proteins: novel molecular targets for cancer drug discovery. Oncogene 19:6613-26 (2000). Lin JX & Leonard WJ The role of Stat5a and Stat5b in signaling by IL-2 family cytokines. Oncogene 19:2566-76 (2000). Horvath CM STAT proteins and transcriptional responses to extracellular signals. Trends Biochem Sci 25:496-502 (2000). Ivashkiv LB Jak-STAT signaling pathways in cells of the immune system. Rev Immunogenet 2:220-30 (2000). |
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