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| Catalog Code: | NB120-7957 |
| Product Name: | Cyclin B1 Antibody |
| Product Description: | Rabbit Polyclonal anti-Cyclin B1 |
| Clonality: | Polyclonal |
| ListPrice: | 295 |
| Concentration: | 200 ug/ml mg/ml |
| Immunogen: | Synthetic peptide: TLPQLNSALVQDLAKAVAKV, corresponding to C terminal amino acids 414-433 of Human Cyclin B1. |
| Specificity: | Reacts with cyclin B1 |
| Cross Reactivity: | Cross-reacts with Human.Not yet tested in other species. |
| Packaging: | 1 ml Each vial contains 0.2 mg/ml of affinity purified rabbit IgG, in 1 ml PBS containing 0.1 % sodium azide and 0.2% gelatin. |
| Uses: | NB120-7957 reacts with cyclin B1 of mouse, rat, and human origin by western blotting and immunohistochemistry. Western blotting starting dilution: 1:200. |
| Localization: | Inactive cyclin B cytoplasmic. |
| Background: | During each cell cycle cyclins undergo periodic accumulation and destruction. As key regulators of the cell cycle the cyclins control important transitions by activating Cdks (1,2). Cyclin A regulates at least two cdks, Cdc2 p34 and Cdk2, and is believed to be necessary for progression through S phase and is active during the G2-M transition (1&3,4). Cyclin B1 also associates with Cdc2 p34 and this complex is requisite for the G2-M progression. The Cyclin B1/Cdc2 p34 complex is known as MPF, M-phase kinase, maturation-promoting factor, or M-phase specific histone kinase, is widely accepted as the trigger of mitosis in all organisms (5-9). |
| Storage: | Store at 4C. Do not freeze. |
| Purity: | IgG purified |
| Isotype: | IgG |
| Host Name: | Rabbit |
| Buffer: | PBS with 0.1% sodium azide |
| Application Summary: | immunohistochemistry-Fr, immunoprecipitation, Western Blot |
| Species Summary: | Hu |
| Alternate Names: | anti-CCNB 1 antibody, anti-CCNB antibody, anti-CCNB1 antibody, anti-G2 mitotic specific cyclin B1 antibody |
| Package Size: | 1 ml |
| General References: | General / background references:Goldstone S et al. Cdc25-dependent activation of cyclin A/cdk2 is blocked in G2 phase arrested cells independently of ATM/ATR. Oncogene 20:921-32 (2001). Liu D et al. A role for cyclin A1 in the activation of MPF and G2-M transition during meiosis of male germ cells in mice. Dev Biol 224:388-400 (2000). Kishimoto T & Okumura E In vivo regulation of the entry into M-phase: initial activation and nuclear translocation of cyclin B/Cdc2. Prog Cell Cycle Res 3:241-9 (1997). Desdouets C et al. Cyclin A: function and expression during cell proliferation. Prog Cell Cycle Res 1:115-23 (1995). Thron CD Theoretical dynamics of the cyclin B-MPF system: a possible role for p13suc1. Biosystems 32:97-109 (1994). Brechot C Oncogenic activation of cyclin A. Curr Opin Genet Dev 3:11-8 (1993). Hoffmann I et al. Phosphorylation and activation of human cdc25-C by cdc2--cyclin B and its involvement in the self-amplification of MPF at mitosis. EMBO J 12:53-63 (1993). Baratte B et al. Screening for antimitotic compounds using the cdc25 tyrosine phosphatase, an activator of the mitosis-inducing p34cdc2/cyclin Bcdc13 protein kinase. Anticancer Res 12:873-80 (1992). Bandara LR et al. Cyclin A recruits p33cdk2 to the cellular transcription factor DRTF1. J Cell Sci Suppl 16:77-85 (1992). |
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