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| Catalog Code: | NB120-7954 |
| Product Name: | Cdk 2 Antibody |
| Product Description: | Rabbit Polyclonal anti-Cdk 2 |
| Clonality: | Polyclonal |
| ListPrice: | 295 |
| Concentration: | 200 ug/ml mg/ml |
| Immunogen: | Synthetic peptide (Human) (C terminal). |
| Specificity: | Reacts with Cdk2 |
| Cross Reactivity: | Cross-reacts with Human, Rat and Mouse.Not yet tested in other species. |
| Packaging: | 1 ml Each vial contains 0.2 mg/ml of affinity purified rabbit IgG, in 1 ml PBS containing 0.1 % sodium azide and 0.2% gelatin. |
| Uses: | NB120-7954 reacts with Cdk2 of mouse, rat, and human origin by western blotting, immunoprecipitation and immunohistochemistry. Western blotting starting dilution: 1:400. |
| Localization: | Cytoplasmic and Nuclear |
| Background: | Cyclin dependent kinases are key regulators of the progression of the cell cycle. Early in the cell cycle Cdk2, Cdk4, Cdk6 and their associated cyclins regulate the G1 to S phase transition (1, 2). Cdk2 plays a key role in the G1/S and S/G2 transitions through its associations with cyclin D1, cyclin D2, cyclin D3, cyclin E and cyclin A. Cdk4 also forms complexes with the D type cyclins, and is thought to regulate cell growth through the G1 phase of the cell cycle (3-6). The late stages of the cell cycle are regulated by another cyclin dependent kinase, Cdc2 p34. This kinase exists as a complex with both cyclin A and cyclin B. The best characterized of these associations is the Cdc2 p34-cyclin B complex that is required for the G2 to M phase transition (7,8). |
| Storage: | Store at 4C. Do not freeze. |
| Purity: | IgG purified |
| Isotype: | IgG |
| Host Name: | Rabbit |
| Buffer: | PBS with 0.1% sodium azide |
| Application Summary: | immunohistochemistry Fr, immunoprecipitation , Western Blot 1:400 |
| Species Summary: | Mu, Rt, Hu |
| Alternate Names: | anti-Cdc2 related protein kinase antibody, anti-Cdk 2 antibody, anti-CDK2 antibody, anti-Cell division kinase 2 antibody, anti-Cell division protein kinase 2 antibody anti-Cyclin dependent kinase 2 antibody, anti-p33 protein kinase antibody, anti-p33(CDK2) antibody |
| Package Size: | 1 ml |
| General References: | General / background references:Chu CY & Lim RW Involvement of p27(kip1) and cyclin D3 in the regulation of cdk2 activity during skeletal muscle differentiation. Biochim Biophys Acta 1497:175-85 (2000). Ekholm SV & Reed SI Regulation of G(1) cyclin-dependent kinases in the mammalian cell cycle. Curr Opin Cell Biol 12:676-84 (2000). Tong W & Pollard JW Progesterone inhibits estrogen-induced cyclin D1 and cdk4 nuclear translocation, cyclin E- and cyclin A-cdk2 kinase activation, and cell proliferation in uterine epithelial cells in mice. Mol Cell Biol 19:2251-64 (1999). Morisaki H et al. Complex mechanisms underlying impaired activation of Cdk4 and Cdk2 in replicative senescence: roles of p16, p21, and cyclin D1. Exp Cell Res 253:503-10 (1999). Kishimoto T & Okumura E In vivo regulation of the entry into M-phase: initial activation and nuclear translocation of cyclin B/Cdc2. Prog Cell Cycle Res 3:241-9 (1997). Sweeney KJ et al. Cyclin D2 activates Cdk2 in preference to Cdk4 in human breast epithelial cells. Oncogene 14:1329-40 (1997). O'Connor PM et al. G2 delay induced by nitrogen mustard in human cells affects cyclin A/cdk2 and cyclin B1/cdc2-kinase complexes differently. J Biol Chem 268:8298-308 (1993). Morla AO et al. Reversible tyrosine phosphorylation of cdc2: dephosphorylation accompanies activation during entry into mitosis. Cell 58:193-203 (1989). |
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