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| Catalog Code: | NB120-7948 |
| Product Name: | ERK 2 Antibody |
| Product Description: | Rabbit Polyclonal anti-ERK 2 |
| Clonality: | Polyclonal |
| ListPrice: | 295 |
| Concentration: | 200 ug/ml mg/ml |
| Immunogen: | Synthetic peptide (Rat) (C terminal). |
| Specificity: | This antibody reacts with ERK 2 p44 and to a lesser extent ERK 1 p42. |
| Cross Reactivity: | Cross-reacts with Human, Rat and Mouse.Not yet tested in other species. |
| Packaging: | 1 ml Each vial contains 0.2 mg/ml of affinity purified rabbit IgG, in 1 ml PBS containing 0.1 % sodium azide and 0.2% gelatin. |
| Uses: | ERK2 (C14) DB020 reacts with ERK2 p42 and to a lesser extent ERK1 p44 of mouse, rat and human origin by western blotting, immunoprecipitation, and immunohistochemistry. Western blotting starting dilution: 1:400. |
| Localization: | Cytoplasmic |
| Background: | The mitogen-activated protein kinases (MAPK) consist of several subgroups, including the ERK, JNK, and p38 kinases. The members of this MAPK family are regulated by many different extracellular cues ranging from cytokines, growth factors, and neuropeptides (1). These stimuli activate cell surface receptors to stresses such as cold, heat, osmolarity changes and irradiation. The pathways regulated by the MAPKs control a broad array of cellular responses ranging from survival, cell proliferation, and apoptosis (1,2). The MAPKs family is also characterized by their requirement for dual phosphorylation at a conserved threonine and tyrosine residue for enzymatic activation and both must be phosphorylated for full enzymatic activation (3). The closely related ERK1 (44 kDa) and ERK2 (42 kDa) kinases are characterized by their requirement for dual phosphorylation at a conserved T-E-Y motif (4,5). While JNK1 is activated by dual phosphorylation at a T-P-Y motif and p38 is also activated by dual phosphorylation at a T-G-Y motif (6,7). |
| Storage: | Store at 4C. Do not freeze. |
| Purity: | IgG purified |
| Isotype: | IgG |
| Host Name: | Rabbit |
| Buffer: | PBS with 0.1% sodium azide |
| Application Summary: | immunohistochemistry P, immunoprecipitation , Western Blot 1:400 |
| Species Summary: | Mu, Rt, Hu |
| Alternate Names: | anti-ERK 2 antibody, anti-ERT 1 antibody, anti-ERT1 antibody, anti-Extracellular Signal Regulated Kinase 2 antibody, anti-MAP kinase 2 antibody, anti-MAPK 1 antibody, anti-MAPK 2 antibody, anti-MAPK1 antibody, anti-MAPK2 antibody, anti-Mitogen Activated Protein Kinase 1 antibody, anti-Mitogen Activated Protein Kinase 2 antibody, anti-P38 antibody, anti-P40 antibody, anti-P41 antibody, anti-P41MAPK antibody, anti-p42 MAPK antibody, anti-P42MAPK antibody, anti-PRKM 1 antibody, anti-PRKM 2 antibody, anti-PRKM1 antibody, anti-PRKM2 antibody, anti-Protein Tyrosine Kinase ERK2 antibody |
| Package Size: | 1 ml |
| General References: | General / background references:Cha H & Shapiro P Tyrosine-phosphorylated extracellular signal--regulated kinase associates with the Golgi complex during G2/M phase of the cell cycle: evidence for regulation of Golgi structure. J Cell Biol 153:1355-67 (2001). Prowse CN & Lew J Mechanism of activation of ERK2 by dual phosphorylation. J Biol Chem 276:99-103 (2001). Fanger GR Regulation of the MAPK family members: role of subcellular localization and architectural organization. Histol Histopathol 14:887-94 (1999). Kyriakis JM Making the connection: coupling of stress-activated ERK/MAPK (extracellular-signal-regulated kinase/mitogen-activated protein kinase) core signalling modules to extracellular stimuli and biological responses. Biochem Soc Symp 64:29-48 (1999). Chan ED et al. Preferential activation of the p46 isoform of JNK/SAPK in mouse macrophages by TNF alpha. Proc Natl Acad Sci U S A 94:13169-74 (1997). Kyriakis JM et al. The stress-activated protein kinase subfamily of c-Jun kinases. Nature 369:156-60 (1994). Nishida E & Gotoh Y The MAP kinase cascade is essential for diverse signal transduction pathways. Trends Biochem Sci 18:128-31 (1993). |
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