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| Catalog Code: | NB120-7947 |
| Product Name: | ERK 1 Antibody |
| Product Description: | Rabbit Polyclonal anti-ERK 1 |
| Clonality: | Polyclonal |
| ListPrice: | 295 |
| Concentration: | 200 ug/ml mg/ml |
| Immunogen: | Synthetic peptide (Rat). Synthetic peptide mapping within the subdomain XI of rat ERK1. |
| Specificity: | ERK1 (I23) DB019 reacts with ERK1 p44 and to a lesser extent ERK2 p42 of mouse, rat, and human origin |
| Cross Reactivity: | Cross-reacts with Human, Rat and Mouse.Not yet tested in other species. |
| Packaging: | 1 ml Each vial contains 0.2 mg/ml of affinity purified rabbit IgG, in 1 ml PBS containing 0.1 % sodium azide and 0.2% gelatin. |
| Localization: | Cytoplasmic |
| Background: | The mitogen-activated protein kinases (MAPK) consist of several subgroups, including the ERK, JNK, and p38 kinases. The members of this MAPK family are regulated by many different extracellular cues ranging from cytokines, growth factors, and neuropeptides (1). These stimuli activate cell surface receptors to stresses such as cold, heat, osmolarity changes and irradiation. The pathways regulated by the MAPKs control a broad array of cellular responses ranging from survival, cell proliferation, and apoptosis (1,2). The MAPKs family is also characterized by their requirement for dual phosphorylation at a conserved threonine and tyrosine residue for enzymatic activation and both must be phosphorylated for full enzymatic activation (3). The closely related ERK1 (44 kDa) and ERK2 (42 kDa) kinases are characterized by their requirement for dual phosphorylation at a conserved T-E-Y motif (4,5). While JNK1 is activated by dual phosphorylation at a T-P-Y motif and p38 is also activated by dual phosphorylation at a T-G-Y motif (6,7). |
| Storage: | Store at 4C. Do not freeze. |
| Purity: | IgG purified |
| Isotype: | IgG |
| Host Name: | Rabbit |
| Buffer: | PBS with 0.1% sodium azide |
| Application Summary: | immunohistochemistry P, immunoprecipitation , Western Blot 1:200 |
| Species Summary: | Mu, Rt, Hu |
| Alternate Names: | anti-ERK 1 antibody, anti-ERK antibody, anti-ERK1 antibody, anti-ERT 2 antibody, anti-ERT2 antibody, anti-Extracellular Signal Regulated Kinase 1 antibody, anti-Extracellular signal related kinase 1 antibody, anti-HGNC6877 antibody, anti-HS44KDAP antibody, anti-HUMKER1A antibody, anti-Insulin Stimulated MAP2 Kinase antibody, anti-MAP kinase 1 antibody, anti-MAP Kinase antibody, anti-MAPK 1 antibody, anti-MAPK 3 antibody, anti-MAPK antibody, anti-MAPK1 antibody, anti-MAPK3 antibody, anti-MGC20180 antibody, anti-Microtubule Associated Protein 2 Kinase antibody, anti-Mitogen Activated Protein Kinase 3 antibody, anti-p44 ERK1 antibody, anti-p44 MAPK antibody, anti-P44ERK1 antibody, anti-P44MAPK antibody, anti-PRKM 3 antibody, anti-PRKM3 antibody, anti-Protein Kinase Mitogen Activated 3 antibody |
| Package Size: | 1 ml |
| General References: | General / background references:Prowse CN & Lew J Mechanism of activation of ERK2 by dual phosphorylation. J Biol Chem 276:99-103 (2001). Cha H & Shapiro P Tyrosine-phosphorylated extracellular signal--regulated kinase associates with the Golgi complex during G2/M phase of the cell cycle: evidence for regulation of Golgi structure. J Cell Biol 153:1355-67 (2001). Fanger GR Regulation of the MAPK family members: role of subcellular localization and architectural organization. Histol Histopathol 14:887-94 (1999). Kyriakis JM Making the connection: coupling of stress-activated ERK/MAPK (extracellular-signal-regulated kinase/mitogen-activated protein kinase) core signalling modules to extracellular stimuli and biological responses. Biochem Soc Symp 64:29-48 (1999). Chan ED et al. Preferential activation of the p46 isoform of JNK/SAPK in mouse macrophages by TNF alpha. Proc Natl Acad Sci U S A 94:13169-74 (1997). Kyriakis JM et al. The stress-activated protein kinase subfamily of c-Jun kinases. Nature 369:156-60 (1994). Nishida E & Gotoh Y The MAP kinase cascade is essential for diverse signal transduction pathways. Trends Biochem Sci 18:128-31 (1993). |
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