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| Catalog Code: | NB120-7336 |
| Product Name: | Plasminogen Antibody |
| Product Description: | Goat Polyclonal anti-Plasminogen, conjugated to HRP |
| Clonality: | Polyclonal |
| ListPrice: | 285 |
| Immunogen: | Plasminogen [Human Plasma] |
| Specificity: | This product is an IgG fraction antibody purified from monospecific antiserum by a multistep process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Goat Serum as well as purified and partially purified Plasminogen [Human Plasma]. Cross reactivity against Plasminogen from other sources is unknown. |
| Cross Reactivity: | Cross-reacts with Human.Not yet tested in other species. |
| Packaging: | 4 mg Stabilizer: 10 mg/ml Bovine Serum Albumin (BSA), IgG and Protease freePreservative: 0.01% (w/v) Gentamicin Sulfate |
| Uses: | ImmunoblottingELISA (1:1,000 to 1:4,000)ImmunohistochemistryImmunoperoxidase electron microscopy as well as other peroxidase-antibody based enzymatic assays. |
| Localization: | Secreted |
| Background: | Plasminogen is a plasma protein synthesised mostly in the liver. Plasma concentration is usually in the range of 70 to 200mg/l. Plasminogen is activated by tissue plasminogen activator, urokinase and streptokinase, to form plasmin. Activation results from the cleavage and release of the preactivation peptide. Angiostatin, an internal fragment of plasminogen, is a potent inhibitor of angiogenesis, which selectively inhibits endothelial cell proliferation. Angiostatin potently inhibits tumor growth and can maintain metastatic and primary tumors in a dormant state. It is defined by a balance of proliferation and apoptosis of the tumor cells and is composed of plasminogen first four cringle structures. This molecule is generated by proteolytic cleavage of plasminogen. |
| Storage: | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze thaw cycles. |
| Purity: | IgG purified |
| Isotype: | IgG |
| Host Name: | Goat |
| Buffer: | 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 |
| Application Summary: | ELISA, immunohistochemistry-Fr, immunofluorescence, Western Blot |
| Species Summary: | Hu |
| Alternate Names: | anti-Angiostatin antibody, anti-Microplasmin antibody, anti-PLG antibody |
| Package Size: | 4 mg |
| Preservative: | 0.01% (w/v) Gentamicin Sulfate |
| Conjugate: | HRP |
| General References: | General / background references:Farr AG & Nakane PK Immunohistochemistry with enzyme labeled antibodies: a brief review. J Immunol Methods 47:129-44 (1981). |
| Novus References: | 1. Basile, D., et al. Angiostatin and matrix metalloprotease expression following ischemic acute renal failure. Am J. Physiol: Renal Physiology. 286:893-902, 2004. |
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