| CatalogCode: | NB120-6583 |
| ProductName: | Collagen Type VI Antibody |
| Product Description: | Rabbit Polyclonal anti-Collagen Type VI, conjugated Biotin |
| Clonality: | Polyclonal |
| Immunogen: | Collagen Type VI from adult human and bovine tissues |
| Specificity: | Negligible cross-reactivity with Type I, II, III, IV or V collagens. Non-specific cross reaction of anti-collagen antibodies with other human serum proteins or non-collagen extracellular matrix proteins is negligible. |
| CrossReactivity: | Cross-reacts with Human and Cow.Expected to cross-react with all Mammals due to sequence homology.Not yet tested in other species. |
| Packaging: | 0.1 mg Liquid |
| Uses: | Immunohistochemistry on frozen tissue sections (1:50 to 1:200) Indirect trapping ELISA (1:4,000 to 1:8,000) for quantitation of antigen in serum using a standard curve. Immunoprecipitation and western blotting (1:5,000 to 1:10,000) for highly sensitive qualitative analysis. Optimal titers for other applications should be determined by the researcher.Greatly diminished reactivity and selectivity of these antibodies will result if denaturing and reducing conditions of SDS-PAGE and immunoblotting are used |
| Localization: | Extracellular matrix. |
| Background: | This antibody is well suited to detect extracellular matrix proteins in normal as well as disease state tissues. Disruption of tissue organization is the hallmark of neoplasia. Malignant lesions can be distinguished from benign by examining the breakdown of basement membranes and loss of 3-dimensional architecture. Malignant cells are presumed to use matrix metalloproteases to degrade barriers created by the extracellular matrix which then allows metastasis to occur. Collagenases, stomelysins and gelatinases can collectively degrade all of the various components of the extracellular matrix, including fibrillar and non-fibrillar collagens and basement membrane glycoproteins. |
| Storage: | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze thaw cycles. |
| Purity: | Immunogen affinity purified |
| Isotype: | IgG |
| Host_Name: | Rabbit |
| Buffer: | 0.01M Na phosphate, 0.25M NaCl, pH7.6, 0.05% Na azide, 0.01% Thimerisol, 15mg/ml BSA |
| ListPrice: | 325 |
| AppSummary: | ELISA, IHC-Fr, IF, IP, WB |
| SpeciesSummary: | Hu, Bv |
| ALTnames: | anti-COL6A1 antibody, anti-Collagen alpha 1(VI) chain antibody, anti-Collagen alpha 1(VI) chain precursor antibody, anti-Collagen type VI alpha 1 antibody, anti-Collagen type VI alpha 2 antibody, anti-Collagen type VI alpha 3 antibody, anti-Collagen VI alpha 1 polypeptide antibody, anti-Collagen VI alpha 2 polypeptide antibody, anti-Collagen VI alpha 3 polypeptide antibody, anti-CollagenVI antibody, anti-Human mRNA for collagen VI alpha 1 C terminal globular domain antibody, anti-OPLL antibody |
| ProteinTarget: | Collagen Type VI |
| PackageSize: | 0.1 mg |
| Conjugate: | Biotin |
| NotesMain: | At least 11 genetically distinct gene products are collectively referred to as 'collagen types' or other proteins and proteoglycans of the extracellular matrix. In humans, collagens are composed of about 20 unique protein chains which under go various types of post-translational modifications and are ultimately assembled into a triple helix. This results in great diversity between collagen types. Collagens are highly conserved throughout evolution and are characterized by an uninterrupted "Glycine-X-Y" triplet repeat that is a necessary part of the triple helical structure. For these reasons it is often extremely difficult to generate antibodies with specificities to collagens. The development of type specific antibodies is dependent on NON-DENATURED three-dimensional epitopes. This preparation results in a native conformation of the protein. |