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| Catalog Code: | NB120-6581 |
| Product Name: | Collagen Type IV Antibody |
| Product Description: | Rabbit Polyclonal anti-Collagen Type IV, conjugated to Biotin |
| Clonality: | Polyclonal |
| ListPrice: | 325 |
| Concentration: | 1.0 mg/ml |
| Immunogen: | Collagen Type IV from human and bovine placenta |
| Specificity: | negligible cross-reactivity with Type I, II, III, V or VI collagens. Non-specific cross reaction of anti-collagen antibodies with other human serum proteins or non-collagen extracellular matrix proteins is negligible. |
| Cross Reactivity: | Cross-reacts with Human and Cow. Expected to cross-react with all Mammals due to sequence homology. Not yet tested in other species. |
| Packaging: | 0.1 mg Immunogen affinity purified Rabbit antisera. |
| Uses: | Immunohistochemistry on frozen tissue sections. Indirect trapping ELISA for quantitation of antigen in serum using a standard curve. Immunoprecipitation and western blotting for highly sensitive qualitative analysis. Optimal titers for other applications should be determined by the researcher. Greatly diminished reactivity and selectivity of these antibodies will result if denaturing and reducing conditions of SDS-PAGE and immunoblotting are used. |
| Localization: | Cell surface (Potential). |
| Background: | These antibodies are well suited to detect extracellular matrix proteins in normal as well as disease state tissues. Disruption of tissue organization is the hallmark of neoplasia. Malignant lesions can be distinguished from benign by examining the breakdown of basement membranes and loss of 3-dimensional architecture. Malignant cells are presumed to use matrix metalloproteases to degrade barriers created by the extracellular matrix which then allows metastasis to occur. Collagenases, stomelysins and gelatinases can collectively degrade all of the various components of the extracellular matrix, including fibrillar and non-fibrillar collagens and basement membrane glycoproteins. |
| Storage: | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze thaw cycles. |
| Purity: | Immunogen affinity purified |
| Isotype: | IgG |
| Host Name: | Rabbit |
| Buffer: | 0.125M Na borate, 0.075M NaCl, pH8.0, 0.005M EDTA, 10 mg/ml BSA |
| Application Summary: | ELISA 1:4000 - 1:8000, immunohistochemistry , immunofluorescence , immunoprecipitation 1:5000 - 1:10,000, Western Blot 1:5000 - 1:10,000, Immunohistochemistry-Frozen 1:50 - 1:200 |
| Species Summary: | Bv, Hu, Ma, |
| Package Size: | 0.1 mg |
| Preservative: | 0.05% Na azide, 0.01% Thimerisol |
| Conjugate: | Biotin |
| Notes Main: | At least 11 genetically distinct gene products are collectively referred to as 'collagen types' or other proteins and proteoglycans of the extracellular matrix. In humans, collagens are composed of about 20 unique protein chains which under go various types of post-translational modifications and are ultimately assembled into a triple helix. This results in great diversity between collagen types. Collagens are highly conserved throughout evolution and are characterized by an uninterrupted "Glycine-X-Y" triplet repeat that is a necessary part of the triple helical structure. For these reasons it is often extremely difficult to generate antibodies with specificities to collagens. The development of type specific antibodies is dependent on NON-DENATURED three-dimensional epitopes. This preparation results in a native conformation of the protein. |
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