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| Catalog Code: | NB120-6401 |
| Product Name: | Cytokeratin Antibody |
| Product Description: | Mouse Monoclonal anti-Cytokeratin (PCK-26) |
| Clone: | PCK-26 |
| Clonality: | Monoclonal |
| ListPrice: | 295 |
| Immunogen: | Full length native protein (purified) (Human epidermis). |
| Specificity: | PCK-26 recognizes the 58 kDa cytokeratin 5, the 56 kDa cytokeratin 6 and the 52 kDa cytokeratin 8 band in immunoblotting (Lane EB et al). It reacts specifically with a wide variety of normal, reactive and neoplastic epithelial tissues. The antibody reacts with simple, cornifying and non-cornifying squamous epithelia and pseudostratified epithelia. It does not react with non-epithelial normal human tissues. |
| Cross Reactivity: | bovine, carp, feline, chicken, canine, goat, guinea pig, human, hamster, lizard, mouse, pig, rabbit, rat, sheep, snake |
| Packaging: | 0.1 ml Mouse ascites. |
| Uses: | AF: Use at an assay dependant dilution. Dot: Use at an assay dependant dilution. IHC-F: Use at an assay dependant dilution. IF: Use at a dilution of 1/300. IHC-P: Use at an assay dependant dilution. IHC-Fr: Use at an assay dependant dilution. WB: Use at an assay dependant dilution. Not tested in other applications.Optimal dilutions/concentrations should be determined by the end user. |
| Localization: | Cytoplasmic |
| Background: | Cytokeratins, a group comprising at least 29 different proteins, are characteristic of epithelial and trichocytic cells. Cytokeratin 5, 6 and 8 are members of the type II neutral-to-basic subfamily.Monoclonal antibodies to cytokeratins are specific markers of epithelial cell differentiation and have been widely used as tools in tumor identification and classification. PCK-26 facilitates the typing of normal, metaplastic and neoplastic cells and it may aid in the discrimination of carcinomas and non-epithelial tumors such as sarcomas, lymphomas and neural tumors. It is also useful in detecting micro-metastases in lymph nodes and other tissues and for determining the origin of poorly differentiated tumors (Lane EB et al,Moll R et al). |
| Storage: | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze thaw cycles. |
| Purity: | Ascites |
| Isotype: | IgG1 |
| Host Name: | Mouse |
| Buffer: | Ascitic fluid with 0.1% sodium azide |
| Application Summary: | immunohistochemistry -P, immunofluorescence 1:300, Western Blot , AF, |
| Species Summary: | Ch, Bv, Gt, Mu, Rb, Rt, Sh |
| Package Size: | 0.1 ml |
| Preservative: | 0.1% sodium azide |
| General References: | This product has been used in:Lane EB et al. A mutation in the conserved helix termination peptide of keratin 5 in hereditary skin blistering. Nature 356:244-6 (1992). Lane EB & Alexander CM Use of keratin antibodies in tumor diagnosis. Semin Cancer Biol 1:165-79 (1990). Moll R et al. The catalog of human cytokeratins: patterns of expression in normal epithelia, tumors and cultured cells. Cell 31:11-24 (1982). |
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