| request information: |
|
| Catalog Code: | NB120-6396 |
| Product Name: | Glycophorins A and B Antibody |
| Product Description: | Mouse Monoclonal anti-Glycophorins A and B (E3) |
| Clone: | E3 |
| Clonality: | Monoclonal |
| ListPrice: | 295 |
| Immunogen: | human thymus |
| Specificity: | Binds to red blood cell glycophorins A & B (glycophorin á and ä chains). Recognizes the non-glycosate homologous regions of these sialoglycoproteins, compromising amino acids 32-35 of Glycophorin B and 61-64 or 64-67 of Glycophorin A. In studies of phenotypically unusual or abnormal erythrocytes, no preferential reactivity with a particular MN or Ss phenotype was shown. In flow cytometry 11.76% of nucleated bone marrow cells, 77.4% of K562 cells and 3% of HEL cells reacted with this antibody. HEMPAS erythrocytes from patients with Paroxysmal Nocturnal Haemoglobinuria are agglutinated by this antibody (Telen MJ et al). |
| Cross Reactivity: | Cross-reacts with Human.Not yet tested in other species. |
| Packaging: | 0.2 mL IgG purified Mouse IgG. |
| Uses: | ELISA: Use at a dilution of 1/6400. FACS: Use at a dilution of 1/400 (10ul per million cells). WB: Use at a dilution of 1/400. Agglutination: Use at a dilution of 1/1000Not tested in other applications.Optimal dilutions/concentrations should be determined by the end user. |
| Background: | Glycophorins A (GYPA) and B (GYPB) are major sialoglycoproteins of the human erythrocyte membrane which bear the antigenic determinants for the MN and Ss blood groups. GYPA gene consists of 7 exons and has 97% sequence homology with GYPB from the 5' UTR to the coding sequence encoding the first 45 amino acids. GYPB accounts for S, s and U specificities. GPA and GPB provide the cells with a large mucin-like surface and it has been suggested this provides a barrier to cell fusion, so minimizing aggregation between red blood cells in the circulation. In addition to the M or N and S or s antigens, that commonly occur in all populations, about 40 related variant phenotypes have been identified. These variants include all the variants of the Miltenberger complex and several isoforms of Sta; also, Dantu, Sat, He, Mg, and deletion variants Ena, S-s-U- and Mk. Most of the variants are resulted from gene recombinations between GYPA and GYPB. These antigens are expressed on early erythroblasts, late erythroblasts, erythroblasts, mature erythrocytes and the cells of erythroid cell lines K562 and HEL, but not on all other cells (mature erythrocytes are characteristically CD235a positive and CD45 and CD71 negative). |
| Storage: | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze thaw cycles. |
| Purity: | IgG purified |
| Isotype: | IgG1 |
| Host Name: | Mouse |
| Buffer: | 50% Glycerol, PBS |
| Application Summary: | flow cytometry / FACS analysis , ELISA , immunofluorescence , Western Blot |
| Species Summary: | Hu, |
| Alternate Names: | anti-CD235 antibody, anti-CD235a antibody, anti-CD235ab antibody, anti-CD235b antibody, anti-PAS2 antibody, anti-PAS3 antibody, anti-S S active sialoglycoprotein antibody, anti-Sialoglycoprotein alpha antibody, anti-Sialoglycoprotein delta antibody |
| Package Size: | 0.2 mL |
| Preservative: | 0.02% Sodium Azide |
| General References: | General / background references:Telen MJ et al. Human erythrocyte antigens. III. Characterization of a panel of murine monoclonal antibodies that react with human erythrocyte and erythroid precursor membranes. Vox Sang 52:236-43 (1987). Telen MJ & Bolk TA Human red cell antigens. IV. The abnormal sialoglycoprotein of Gerbich-negative red cells. Transfusion 27:309-14 (1987). |
order or
more information: | company product webpage |
|
|