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| Catalog Code: | NB120-6323 |
| Product Name: | COP beta Antibody |
| Product Description: | Mouse Monoclonal anti-COP beta (maD) |
| Clone: | maD |
| Clonality: | Monoclonal |
| ListPrice: | 295 |
| Immunogen: | Synthetic peptide conjugated to KLH, corresponding to amino acids 701-715 of COP (beta). |
| Specificity: | Recognizes an epitope in the beta-COP protein (110kD). The antibody stains the periphery of the Golgi complex in immunohistology using cultured cells. |
| Cross Reactivity: | Cross-reacts with Hamster, Human, Rat and Monkey. Not yet tested in other species. |
| Packaging: | 0.1 ml of Mouse ascites. |
| Uses: | IF: Use at a dilution of 1/80 using methanol/acetone or paraformaldehyde fixation, followed by a permeabilization step. Strong immunofluorescent staining is confined mainly to the central perinuclear area in the cell. May be used for micro-injection into cells. |
| Storage: | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze thaw cycles. |
| Purity: | Ascites |
| Isotype: | IgG1 |
| Host Name: | Mouse |
| Application Summary: | immunohistochemistry Use at an assay dependent dilution, immunofluorescence 1/80, Western Blot 1/1000, Immunocytochemistry Use at an assay dependent dilution, AF: Use at an assay dependent dilution |
| Species Summary: | Ha, Hu, Mk, Rt, |
| Package Size: | 0.1 ml |
| Preservative: | 15mM Sodium Azide |
| General References: | General / background references:Kreis TE et al. COPs regulating membrane traffic. Annu Rev Cell Dev Biol 11:677-706 (1995). Griffiths G et al. Immunocytochemical localization of beta-COP to the ER-Golgi boundary and the TGN. J Cell Sci 108 (Pt 8):2839-56 (1995). Whitney JA et al. Cytoplasmic coat proteins involved in endosome function. Cell 83:703-13 (1995). Pepperkok R et al. Beta-COP is essential for biosynthetic membrane transport from the endoplasmic reticulum to the Golgi complex in vivo. Cell 74:71-82 (1993). Oprins A et al. Beta-COP localizes mainly to the cis-Golgi side in exocrine pancreas. J Cell Biol 121:49-59 (1993). Narula N et al. Identification of a 200-kD, brefeldin-sensitive protein on Golgi membranes. J Cell Biol 117:27-38 (1992). Duden R et al. Beta-COP, a 110 kd protein associated with non-clathrin-coated vesicles and the Golgi complex, shows homology to beta-adaptin. Cell 64:649-65 (1991). Ktistakis NT et al. PtK1 cells contain a nondiffusible, dominant factor that makes the Golgi apparatus resistant to brefeldin A. J Cell Biol 113:1009-23 (1991). Donaldson JG et al. Dissociation of a 110-kD peripheral membrane protein from the Golgi apparatus is an early event in brefeldin A action. J Cell Biol 111:2295-306 (1990). Allan VJ & Kreis TE A microtubule-binding protein associated with membranes of the Golgi apparatus. J Cell Biol 103:2229-39 (1986). |
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