| CatalogCode: | NB120-6317 |
| ProductName: | Cdk 6 Antibody |
| Product Description: | Mouse Monoclonal anti-Cdk 6 (DCS-90) |
| Clone: | DCS-90 |
| Clonality: | Monoclonal |
| Immunogen: | Recombinant full length protein (Human). |
| Specificity: | Reacts specifically with Cdk6. Does not recognize other Cdk types. |
| CrossReactivity: | Cross-reacts with Human, Mouse and Rat.Not yet tested in other species. |
| Packaging: | 0.1 ml Mouse ascites. |
| Uses: | ICC: Use at an assay dependant dilution. IP: Use at an assay dependant dilution. WB: Use at a dilution of 1/1000. (using a cultured human tumour cell line extract. Band at 40kD, with an additional band at approx. 60kD)Not tested in other applications.Optimal dilutions/concentrations should be determined by the end user. |
| Localization: | Cytoplasmic and Nuclear. |
| Background: | Cdk6 is a member of the cyclin-dependent protein kinase (CDK) family. CDK family members are highly similar to the gene products of Saccharomyces cerevisiae cdc28, and Schizosaccharomyces pombe cdc2, and are known to be important regulators of cell cycle progression. This kinase is a catalytic subunit of the protein kinase complex that is important for cell cycle G1 phase progression and G1/S transition. The activity of Cdk6 first appears in mid-G1 phase, and is controlled by regulatory subunits including D-type cyclins and members of INK4 family of CDK inhibitors. This kinase, as well as CDK4, has been shown to phosphorylate, and thus regulate the activity of, tumor suppressor protein Rb. |
| Storage: | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze thaw cycles. |
| Purity: | Ascites |
| Isotype: | IgG1 |
| Host_Name: | Mouse |
| Buffer: | Ascitic fluid with 15mM sodium azide |
| ListPrice: | 330 |
| AppSummary: | ICC, IP, WB |
| SpeciesSummary: | Hu, Rt, Mu |
| ALTnames: | anti-Serine/threonine protein kinase PLSTIRE antibody, anti-CDK 6 antibody, anti-CDK6 antibody, anti-Cell division protein kinase 6 antibody, anti-Crk2 antibody, anti-Cyclin dependent kinase 6 antibody, anti-MGC59692 antibody, anti-p40 antibody, anti-PLSTIRE antibody, anti-Serine/threonine-protein kinase PLSTIRE antibody |
| ProteinTarget: | Cdk 6 |
| PackageSize: | 0.1 ml |
| GeneralRef: | General / background references:Elledge SJ Cell cycle checkpoints: preventing an identity crisis. Science 274:1664-72 (1996). Bates S et al. CDK6 (PLSTIRE) and CDK4 (PSK-J3) are a distinct subset of the cyclin-dependent kinases that associate with cyclin D1. Oncogene 9:71-9 (1994). Meyerson M & Harlow E Identification of G1 kinase activity for cdk6, a novel cyclin D partner. Mol Cell Biol 14:2077-86 (1994). Freeman RS & Donoghue DJ Protein kinases and protooncogenes: biochemical regulators of the eukaryotic cell cycle. Biochemistry 30:2293-302 (1991). Pines J & Hunter T Human cyclins A and B1 are differentially located in the cell and undergo cell cycle-dependent nuclear transport. J Cell Biol 115:1-17 (1991). Yamashita M et al. Developmental Growth and Differentiation 33:617 (0). |
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