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| Catalog Code: | NB120-6301 |
| Product Name: | Catenin beta Antibody |
| Product Description: | Mouse Monoclonal anti-Catenin beta (15B8) |
| Clone: | 15B8 |
| Clonality: | Monoclonal |
| ListPrice: | 295 |
| Immunogen: | Recombinant full length protein (Chicken). |
| Specificity: | Recognizes beta-catenin in immunoblotting (94kD). Does not recognize plakoglobin. |
| Cross Reactivity: | Cross-reacts with Cow, Dog and Human.Not yet tested in other species. |
| Packaging: | 0.1 ml |
| Uses: | ICC: Use at an assay dependant dilution. IF: Use at a dilution of 1/1000. Western Blot: Use at a dilution of 1/1000. Optimal dilutions/concentrations should be determined by the end user. |
| Localization: | Cytoplasmic when it is unstabilized (high level of phosphorylation) or bound to CDH1. Translocates to the nucleus when it is stabilized (low level of phosphorylation). |
| Background: | The distinct peripheral cytosolic proteins, alpha, beta and gamma catenin (102, 94 and 86 kDa) are found in many tissues bind to the conserved cytoplasmic tail domain of the cell adhesion cadherins. Catenins link E cadherin to other integral membrane or cytoplasmic proteins and are modulated by Wnt1 proto oncogene. The central core region of beta catenin is involved in mediation of cadherin catenin complex interaction with EGFR. Beta-Catenin-mediated signalling is involved at several stages of vertebrate neural development. |
| Storage: | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze thaw cycles. |
| Purity: | Ascites |
| Isotype: | IgG1 |
| Host Name: | Mouse |
| Buffer: | Ascitic fluid with 0.1% sodium azide |
| Application Summary: | immunofluorescence 1:1000, Western Blot, Gel Super Shift Assays, Immunocytochemistry, |
| Species Summary: | Hu, Ca, Bv |
| Alternate Names: | anti-Beta catenin antibody, anti-Cadherin associated protein antibody, anti-Catenin beta 1 antibody, anti-CATNB antibody, anti-CTNNB antibody, anti-CTNNB1 antibody |
| Package Size: | 0.1 ml |
| Preservative: | 0.1% sodium azide |
| General References: | General / background references:Munemitsu S et al. Regulation of intracellular beta-catenin levels by the adenomatous polyposis coli (APC) tumor-suppressor protein. Proc Natl Acad Sci U S A 92:3046-50 (1995). Schäfer S et al. Identification of the ubiquitous human desmoglein, Dsg2, and the expression catalogue of the desmoglein subfamily of desmosomal cadherins. Exp Cell Res 211:391-9 (1994). Shiozaki H et al. Immunohistochemical detection of alpha-catenin expression in human cancers. Am J Pathol 144:667-74 (1994). Hoschuetzky H et al. Beta-catenin mediates the interaction of the cadherin-catenin complex with epidermal growth factor receptor. J Cell Biol 127:1375-80 (1994). Reynolds AB et al. Identification of a new catenin: the tyrosine kinase substrate p120cas associates with E-cadherin complexes. Mol Cell Biol 14:8333-42 (1994). Cowin P Unraveling the cytoplasmic interactions of the cadherin superfamily. Proc Natl Acad Sci U S A 91:10759-61 (1994). Tsukita S et al. Submembranous junctional plaque proteins include potential tumor suppressor molecules. J Cell Biol 123:1049-53 (1993). Bradley RS et al. Expression of Wnt-1 in PC12 cells results in modulation of plakoglobin and E-cadherin and increased cellular adhesion. J Cell Biol 123:1857-65 (1993). Su LK et al. Association of the APC tumor suppressor protein with catenins. Science 262:1734-7 (1993). Ozawa M et al. Uvomorulin-catenin complex formation is regulated by a specific domain in the cytoplasmic region of the cell adhesion molecule. Proc Natl Acad Sci U S A 87:4246-50 (1990). Nagafuchi A & Takeichi M Transmembrane control of cadherin-mediated cell adhesion: a 94 kDa protein functionally associated with a specific region of the cytoplasmic domain of E-cadherin. Cell Regul 1:37-44 (1989). Ozawa M et al. The cytoplasmic domain of the cell adhesion molecule uvomorulin associates with three independent proteins structurally related in different species. EMBO J 8:1711-7 (1989). |
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