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| Catalog Code: | NB120-6300 |
| Product Name: | Catenin beta Antibody |
| Product Description: | Mouse Monoclonal anti-Catenin beta (6F9) |
| Clone: | 6F9 |
| Clonality: | Monoclonal |
| ListPrice: | 295 |
| Immunogen: | Recombinant full length protein (Chicken). |
| Specificity: | Recognizes beta-catenin (94kD) in immunoblotting. Does not cross-react with plakoglobin. |
| Cross Reactivity: | Cross-reacts with Cow, Dog and Human.Not yet tested in other species. |
| Packaging: | 0.1 ml |
| Uses: | ICC: Use at an assay dependant dilution. IF: Use at a dilution of 1/1000. Western Blot: Use at a dilution of 1/1000. Optimal dilutions/concentrations should be determined by the end user. |
| Localization: | Cytoplasmic when it is unstabilized (high level of phosphorylation) or bound to CDH1. Translocates to the nucleus when it is stabilized (low level of phosphorylation). |
| Background: | The distinct peripheral cytosolic proteins, alpha, beta and gamma catenin (102, 94 and 86 kDa) are found in many tissues bind to the conserved cytoplasmic tail domain of the cell adhesion cadherins. Catenins link E cadherin to other integral membrane or cytoplasmic proteins and are modulated by Wnt1 proto oncogene. The central core region of beta catenin is involved in mediation of cadherin catenin complex interaction with EGFR. Beta-Catenin-mediated signalling is involved at several stages of vertebrate neural development. |
| Storage: | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze thaw cycles. |
| Purity: | Ascites |
| Isotype: | IgG1 |
| Host Name: | Mouse |
| Buffer: | Ascitic fluid with 0.1% sodium azide |
| Application Summary: | Immunocytochemistry, immunofluorescence, Western Blot, |
| Species Summary: | Hu, Ca, Bv |
| Alternate Names: | anti-Beta catenin antibody, anti-Cadherin associated protein antibody, anti-Catenin beta 1 antibody, anti-CATNB antibody, anti-CTNNB antibody, anti-CTNNB1 antibody |
| Package Size: | 0.1 ml |
| Preservative: | 0.1% sodium azide |
| General References: | General / background references:Reynolds AB et al. Identification of a new catenin: the tyrosine kinase substrate p120cas associates with E-cadherin complexes. Mol Cell Biol 14:8333-42 (1994). Tsukita S et al. Submembranous junctional plaque proteins include potential tumor suppressor molecules. J Cell Biol 123:1049-53 (1993). Johnson KR et al. P- and E-cadherin are in separate complexes in cells expressing both cadherins. Exp Cell Res 207:252-60 (1993). Edelman GM & Crossin KL Cell adhesion molecules: implications for a molecular histology. Annu Rev Biochem 60:155-90 (1991). Takeichi M Cadherin cell adhesion receptors as a morphogenetic regulator. Science 251:1451-5 (1991). Nagafuchi A & Takeichi M Cell binding function of E-cadherin is regulated by the cytoplasmic domain. EMBO J 7:3679-84 (1988). |
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