| CatalogCode: | | NB120-6277 |
| ProductName: | | Actin beta Antibody |
| Product Description: | | Mouse Monoclonal anti-Actin beta (AC-15), conjugated to FITC |
| Clone: | | AC-15 |
| Clonality: | | Monoclonal |
| Immunogen: | | Synthetic peptide: DDDIAALVIDNGSGL conjugated to KLH, corresponding to amino acids 1-14 of Xenopus laevis Actin (beta). |
| Epitope: | | The N-terminal of the beta isoform of actin |
| CrossReactivity: | | Cross-reacts with Cat, Chicken, Cow, Dog, Drosophila melanogaster, Fish (carp), Guinea Pig, Human, Leech, Mouse, Pig, Rabbit, Rat and Sheep. Not yet tested in other species. Cross-reacts in a wide variety of tissues and species. Does not cross-react with adult cardiac or skeletal muscle or amoeba beta actin. |
| Packaging: | | 0.1 ml IgG purified Mouse ascites. |
| Uses: | | IF: Use for labelling of cultured human and chicken fibroblasts.Optimal dilutions/concentrations should be determined by the end user. |
| Localization: | | Cytoplasmic |
| Control: | | Cultured human or chicken fibroblasts. Molecular weight of beta actin is 42Kd. |
| Background: | | Useful for direct immunofluoresence labelling of the cytoskeleton in cultured cells |
| Storage: | | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze thaw cycles. |
| Purity: | | IgG purified |
| Isotype: | | IgG1 |
| Host_Name: | | Mouse |
| Buffer: | | PBS with 1% BSA |
| ListPrice: | | 325 |
| AppSummary: | | IF, ICC |
| SpeciesSummary: | | Bv, Ca, Ch, Dr, Hu, Mu, Po, Rt, Rb, Gp, Fe, Fi, Sh |
| ALTnames: | | Anti-Actin cytoplasmic 1 antibody; Anti-Beta cytoskeletal actin antibody |
| ProteinTarget: | | Actin (beta) |
| PackageSize: | | 0.1 ml |
| Conjugate: | | FITC |
| GeneralRef: | | This product has been used in:Macpherson GR et al. Anti-angiogenic activity of human endostatin is HIF-1-independent in vitro and sensitive to timing of treatment in a human saphenous vein assay. Mol Cancer Ther 2:845-54 (2003). Pingoud-Meier C et al. Loss of caspase-8 protein expression correlates with unfavorable survival outcome in childhood medulloblastoma. Clin Cancer Res 9:6401-9 (2003). Gimona M et al. Beta-actin specific monoclonal antibody. Cell Motil Cytoskeleton 27:108-16 (1994). North AJ et al. Actin isoform compartments in chicken gizzard smooth muscle cells. J Cell Sci 107 (Pt 3):445-55 (1994). North AJ et al. Calponin is localised in both the contractile apparatus and the cytoskeleton of smooth muscle cells. J Cell Sci 107 (Pt 3):437-44 (1994). General / background references:Drew JS et al. Localization of isoactins in isolated smooth muscle thin filaments by double gold immunolabeling. Am J Physiol 260:C1332-40 (1991). Lessard JL Two monoclonal antibodies to actin: one muscle selective and one generally reactive. Cell Motil Cytoskeleton 10:349-62 (1988). Vandekerckhove J & Weber K Actin amino-acid sequences. Comparison of actins from calf thymus, bovine brain, and SV40-transformed mouse 3T3 cells with rabbit skeletal muscle actin. Eur J Biochem 90:451-62 (1978). |
| NotesMain: | | Immunofluoresence staining of chicken gizzard ultra-thin sections labels the dense bodies, longitudinal channels and membrane associated dense-plaque. |
| more information: | | company product webpage |