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| Catalog Code: | NB120-6158 |
| Product Name: | IRAK 2 Antibody |
| Product Description: | Rabbit Polyclonal anti-IRAK 2 |
| Clonality: | Polyclonal |
| ListPrice: | 295 |
| Immunogen: | Synthetic peptide, corresponding to amino acids 571-590 of Human IRAK2. |
| Specificity: | This antibody reacts with the human IRAK2 protein. On Western blots, it identifies the target band at ~65 kDa. Another band at ~130 kDa may represent a dimer. In K562 cell lysates, a band at ~80 kDa has also been observed. Anti-IRAK2 has no crossresponse to IRAK. |
| Cross Reactivity: | Cross-reacts with Human.Not yet tested in other species. |
| Packaging: | 0.1 mg |
| Uses: | Western blot at 1:500 to 1:1000 dilution and a65 kDa band can be detected. |
| Background: | Nuclear factor kappa B (NF-kB) is a ubiquitous transcription factor and an essential mediator of gene expression during activation of immune and inflammatory responses. NF-kB mediates the expression of a great variety of genes in response to extracellular stimuli including IL-1, TNFa, and bacteria product LPS. NF-kB is associated with IkB proteins in the cell cytoplasm, which inhibit NF-kB activity. The long-sought IkB kinase (IKK),which phosphorylates IkB, and mediates IkB degradation and NF-kB activation, was recently identified by several laboratories (1-5). IKK is a serine protein kinase, and the IKK complex contains alpha and beta subunits (IKKa and IKKb). IKKa and IKKb interact with each other and both are essential for NF-kB activation. IKKb phosphorylates both IkB-a and IkB-b. IKKb is expressed in a variety of human tissues. |
| Storage: | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze thaw cycles. |
| Purity: | IgG purified |
| Isotype: | IgG |
| Host Name: | Rabbit |
| Buffer: | PBS with 0.02% sodium azide |
| Application Summary: | Western Blot |
| Species Summary: | Hu |
| Alternate Names: | anti-Interleukin 1 receptor associated kinase like 2 antibody, anti-Interleukin 1 receptor associated kinase 2 antibody, anti-Interleukin 1 receptor-associated kinase like 2 antibody, anti-IRAK 2 antibody, anti-IRAK2 antibody, anti-MGC150550 antibody |
| Package Size: | 0.1 mg |
| General References: | General / background references:Zhang FX et al. Bacterial lipopolysaccharide activates nuclear factor-kappaB through interleukin-1 signaling mediators in cultured human dermal endothelial cells and mononuclear phagocytes. J Biol Chem 274:7611-4 (1999). Yang RB et al. Signaling events induced by lipopolysaccharide-activated toll-like receptor 2. J Immunol 163:639-43 (1999). Muzio M et al. IRAK (Pelle) family member IRAK-2 and MyD88 as proximal mediators of IL-1 signaling. Science 278:1612-5 (1997). Cao Z et al. IRAK: a kinase associated with the interleukin-1 receptor. Science 271:1128-31 (1996). |
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