| request information: |
|
| Catalog Code: | NB120-6148 |
| Product Name: | IRAK 2 Antibody |
| Product Description: | Rabbit Polyclonal anti-IRAK 2 |
| Clonality: | Polyclonal |
| ListPrice: | 310 |
| Immunogen: | Synthetic peptide, corresponding to amino acids 546-564 of Human IRAK2. |
| Specificity: | This antibody reacts with the human IRAK2 protein. On Western blots, it identifies the target band at ~65 kDa. Another band at ~130 kDa may represent a dimer. In K562 cell lysates, a band at ~80 kDa has also been observed. Anti-IRAK2 has no cross response to IRAK. |
| Cross Reactivity: | Cross-reacts with Human.Not yet tested in other species. |
| Packaging: | 0.1 mg purified Rabbit IgG. |
| Uses: | Western blot at 1:500 to 1:1000 dilution and a 65 kDa band should be detected. |
| Background: | The pro-inflammatory cytokine IL-1 induces cellular response through two subunits of its receptor, IL-1 receptor I (IL-1RI) and IL-1 receptor accessory protein (IL-1RAcP). IL-1 receptor-associated kinase (IRAK) mediates activation of NF-kB, which is a pivotal transcription factor mediating inflammatory and immune response. A novel member in the IRAK/Pelle family was recently identified and designated IRAK2 (1). Both IRAK and IRAK2 recruit to the subunits of the IL-1R complex after IL-1 binding and lead to NF-kB activation. IRAKs also associate with Toll-like receptor (TLR) and the dominant negative mutants of IRAKs inhibit LPS-induced NF-kB activation (2,3).Members in IRAK/Pelle family play a central role in IL-1R and TLR mediated inflammatory response. IRAK2 is expressed in a variety of human tissues. |
| Storage: | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze thaw cycles. |
| Purity: | IgG purified |
| Isotype: | IgG |
| Host Name: | Rabbit |
| Buffer: | PBS with 0.02% sodium azide |
| Application Summary: | Western Blot 1-3 ìg/mL |
| Species Summary: | Hu |
| Alternate Names: | anti-Interleukin 1 receptor associated kinase like 2 antibody, anti-Interleukin 1 receptor associated kinase 2 antibody, anti-Interleukin 1 receptor-associated kinase like 2 antibody, anti-IRAK 2 antibody, anti-IRAK2 antibody, anti-MGC150550 antibody |
| Package Size: | 0.1 mg |
| General References: | General / background references:Zhang FX et al. Bacterial lipopolysaccharide activates nuclear factor-kappaB through interleukin-1 signaling mediators in cultured human dermal endothelial cells and mononuclear phagocytes. J Biol Chem 274:7611-4 (1999). Yang RB et al. Signaling events induced by lipopolysaccharide-activated toll-like receptor 2. J Immunol 163:639-43 (1999). Muzio M et al. IRAK (Pelle) family member IRAK-2 and MyD88 as proximal mediators of IL-1 signaling. Science 278:1612-5 (1997). |
order or
more information: | company product webpage |