| CatalogCode: | NB120-6146 |
| ProductName: | IKK beta Antibody |
| Product Description: | Rabbit Polyclonal anti-IKK beta |
| Clonality: | Polyclonal |
| Immunogen: | Synthetic peptide corresponding to amino acids 662-680 of human IKK beta (3,4), which differs from correspondingmurine sequence by one amino acid (5). |
| Specificity: | Recognize IKK beta and does not cross react with IKK alpha or IKK gamma. |
| CrossReactivity: | Cross-reacts with Human.Not yet tested in other species. |
| Packaging: | 0.1 mg Purified IgG - liquid |
| Uses: | Suggested working dilution(s): western blotting - 1/500 - 1/1000 |
| Localization: | Cytoplasmic |
| Control: | Whole celllysate from Jurkat cells. |
| Background: | Nuclear factor kappa B (NF-kB) is a ubiquitous transcription factor and an essential mediator of gene expression during activation of immune and inflammatory responses. NF-kB mediates the expression of a great variety of genes in response to extracellular stimuli including IL-1, TNFa, and bacteria product LPS. NF-kB is associated with IkB proteins in the cell cytoplasm, which inhibit NF-kB activity. The long-sought IkB kinase (IKK),which phosphorylates IkB, and mediates IkB degradation and NF-kB activation, was recently identified by several laboratories (1-5). IKK is a serine protein kinase, and the IKK complex contains alpha and beta subunits (IKKa and IKKb). IKKa and IKKb interact with each other and both are essential for NF-kB activation. IKKb phosphorylates both IkB-a and IkB-b. IKKb is expressed in a variety of human tissues. |
| Storage: | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze thaw cycles. |
| Purity: | IgG purified |
| Isotype: | IgG |
| Host_Name: | Rabbit |
| Buffer: | PBS with 0.02% sodium azide |
| ListPrice: | 330 |
| AppSummary: | WB |
| SpeciesSummary: | Hu |
| ProteinTarget: | IKK beta |
| PackageSize: | 0.1 mg |
| GeneralRef: | General / background references:Nakano H et al. Differential regulation of IkappaB kinase alpha and beta by two upstream kinases, NF-kappaB-inducing kinase and mitogen-activated protein kinase/ERK kinase kinase-1. Proc Natl Acad Sci U S A 95:3537-42 (1998). DiDonato JA et al. A cytokine-responsive IkappaB kinase that activates the transcription factor NF-kappaB. Nature 388:548-54 (1997). Regnier CH et al. Identification and characterization of an IkappaB kinase. Cell 90:373-83 (1997). Zandi E et al. The IkappaB kinase complex (IKK) contains two kinase subunits, IKKalpha and IKKbeta, necessary for IkappaB phosphorylation and NF-kappaB activation. Cell 91:243-52 (1997). Woronicz JD et al. IkappaB kinase-beta: NF-kappaB activation and complex formation with IkappaB kinase-alpha and NIK. Science 278:866-9 (1997). Mercurio F et al. IKK-1 and IKK-2: cytokine-activated IkappaB kinases essential for NF-kappaB activation. Science 278:860-6 (1997). |