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| Catalog Code: | NB120-5989 |
| Product Name: | PHAP Antibody |
| Product Description: | Rabbit Polyclonal anti-PHAP |
| Clonality: | Polyclonal |
| ListPrice: | 295 |
| Concentration: | 1 mg/ml |
| Immunogen: | Synthetic peptide (Human). |
| Specificity: | This antibody cross-reacts to all three identified isoforms of PHAP (PHAPI, PHAP12a and PHAPIII). |
| Cross Reactivity: | Cross-reacts with Human, Mouse and Rat.Not yet tested in other species. |
| Packaging: | 0.05 mg Immunogen affinity purified Rabbit antisera. |
| Uses: | In Western Blot detects a band of approximately 35 kDa corresponding to PHAPIII. A band below 35kDa is also observed, consisting of a doublet of PHAPI and PHAP12a. Not tested in other applications.Optimal dilutions/concentrations should be determined by the end user. |
| Localization: | Cytoplasmic and Nuclear |
| Background: | This antibody recognizes all three identified isoforms of human, mouse and rat PHAP, including PHAP I, PHAP I2a, and PHAP III. The PHAP proteins(tumor suppressor putative HLA-DR associated proteins) are important regulators of mitochondrial apoptosis. PHAP facilitates apoptosome-mediated caspase-9 activation to stimulate the mitochondrial apoptosis pathway. In addition, PHAP opposes both Ras- and myc- mediated cell transformation. Apoptosis is related to many diseases and development. Caspase 9 plays a central role in cell death induced by a variety of apoptosis activators. Cytochrome c, after released from mitochondria, binds to Apaf 1, which forms an apoptosome that in turn binds to and activate procaspase 9. Activated caspase 9 cleaves and activates the effector caspases (caspase 3, 6 and 7), which are responsible for the proteolytic cleavage of many key proteins in apoptosis. The tumor suppressor putative HLA-DR-associated proteins (PHAPs) were recently identified as important regulators of mitochondrion apoptosis. PHAP appears to facilitate apoptosome-mediated caspase 9 activation and to stimulate the mitochondrial apoptotic pathway. PHAP was also shown to oppose both Ras- and Myc-medicated cell transformation. |
| Storage: | Store at +4°C. |
| Purity: | Immunogen affinity purified |
| Isotype: | IgG |
| Host Name: | Rabbit |
| Buffer: | PBS |
| Application Summary: | Western Blot 0.5 to 2 ìg/ml |
| Species Summary: | Hu, Mu, Rt, |
| Alternate Names: | anti-LANP antibody, anti-PHAP1 antibody |
| Package Size: | 0.05 mg |
| Preservative: | 0.02% Sodium Azide |
| Notes Main: | Stable for one year. |
| General References: | General / background references:Jiang X et al. Distinctive roles of PHAP proteins and prothymosin-alpha in a death regulatory pathway. Science 299:223-6 (2003). Nicholson DW & Thornberry NA Apoptosis. Life and death decisions. Science 299:214-5 (2003). |
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