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| Catalog Code: | NB120-5908 |
| Product Name: | IP3R1 Antibody |
| Product Description: | Rabbit Polyclonal anti-IP3R1 |
| Clonality: | Polyclonal |
| ListPrice: | 295 |
| Immunogen: | Synthetic peptide: KKKDDEVDRDAPSRKKAKE, corresponding to amino acids 1829-1848 of Human IP3R1. This sequence is 100% conserved in human, mouse, and rat IP3R-I protein. |
| Cross Reactivity: | Cross-reacts with Rat and Dog. Expected to cross-react with Human (100% identity with immunogen) and Mouse (100% identity with immunogen) due to sequence homology.Not yet tested in other species. |
| Packaging: | 0.1 ml Whole Rabbit antisera. |
| Uses: | IHC: Use at a dilution of 1/100. Immunohistochemical staining of IP3R-I in rat cerebellum with ab5908 results in staining of Purkinje cells.IP: Use at an assay dependent dilution. WB: Use at a dilution of 1/1000. Detects a band of approximately 240 kDa (predicted molecular weight: 323 kDa) representing IP3R-I from rat brain extract. |
| Localization: | Endoplasmic reticulum - Integral membrane protein. |
| Background: | Inositol 1,4,5-trisphosphate (IP3) is a second messenger for many growth factors, hormones, and neuro-transmitters. Upon binding to the IP3 receptor (IP3R), IP3 triggers the release of intracellular, luminal calcium to the cytosol. Functional IP3R is a homo- or heterotetramer of ~240 kDa glycoprotein subunits, that is structurally reminiscent of members of the voltage-gated ion channel superfamily. IP3R protein is structurally and functionally related to one other important intracellular calcium-release channel, the ryanodine receptor. The similarity between the two receptors continues at the physiological level owing to a physical association each receptor can have with the immunophilin protein, FKBP12. Cytosolic calcium levels appear to be regulated by a feedback loop that starts with calcium activation of protein kinase C (PKC) and calcineurin, a protein phosphatase. Phosphorylation of the IP3R by PKC causes an increase in IP3-mediated calcium release. Concomitantly, the phosphatase activity of calcineurin is stimulated upon its association with the FKBP12-IP3R complex. Calcium release is reduced when the PKC target site on the IP3R is dephosphorylated by calcineurin resulting in calcium oscillations. Mammalian IP3R subunits are the product of three distinct genes that are widely expressed and differentially regulated. Type-I IP3R has been detected in heart, liver, kidney, ovary, and Purkinje neurons of the cerebellum. IP3R-II protein is found predominantly in the brain. Isoform III expression has been shown to occur in pancreatic islets, kidney and the gastrointestinal tract. |
| Purity: | Whole antisera |
| Isotype: | IgG |
| Host Name: | Rabbit |
| Buffer: | Constituents: PBS |
| Application Summary: | immunohistochemistry, immunoprecipitation, Western Blot |
| Species Summary: | Ca, Hu, Mu, Rt, |
| Alternate Names: | anti-IP3, anti- Anti-IP3R, anti- Anti-IP3R1, anti- Anti-ITPR1, anti- Anti-Inositol 145-trisphosphate Receptor Type 1, anti- IP3, anti- IP3R, anti- IP3R1, anti- ITPR1, anti- Inositol 145-trisphosphate Receptor Type 1 antibody |
| Package Size: | 0.1 ml |
| Preservative: | 0.05% Sodium Azide |
| General References: | General / background references:Dean WL & Quinton TM Distribution of plasma membrane Ca(2+)-ATPase and inositol 1,4,5-trisphosphate receptor in human platelet membranes. Cell Calcium 17:65-70 (1995). Cameron AM et al. Calcineurin associated with the inositol 1,4,5-trisphosphate receptor-FKBP12 complex modulates Ca2+ flux. Cell 83:463-72 (1995). Taylor CW & Traynor D Calcium and inositol trisphosphate receptors. J Membr Biol 145:109-18 (1995). |
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