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| Catalog Code: | NB120-5903 |
| Product Name: | Cyclophilin 40 Antibody |
| Product Description: | Rabbit Polyclonal anti-Cyclophilin 40 |
| Clonality: | Polyclonal |
| ListPrice: | 285 |
| Immunogen: | Synthetic peptide: GIGHTTGKPLHFKGC, corresponding to amino acids 57-71 of Human Cyclophilin 40. |
| Specificity: | NB120-5903 detects cyclophilin 40 (CyP-40) from human and rat tissues and cells. |
| Cross Reactivity: | Cross-reacts with Human and Rat.Expected to cross-react with Cow (93% identity with immunogen) and Mouse (86% identity with immunogen) due to sequence homology.Not yet tested in other species. |
| Packaging: | 0.1 ml Whole Rabbit antisera. |
| Uses: | WB: Use at a dilution of 1/500. Detects a band of approximately 40 kDa (predicted molecular weight: 44 kDa) representing CyP-40 from rat brain extract. Not tested in other applications.Optimal dilutions/concentrations should be determined by the end user. |
| Localization: | Cytoplasmic |
| Background: | Immunophilins are a family of soluble cytosolic receptors capable of binding to one of two major immunosuppressant agents: cyclosporin A (CsA) or FK506. Proteins that bind FK506 are termed FK506 Binding Proteins (FKBPs) and those that bind cyclosporin A are called cyclophilins (CyP). Both CyP:CsA and FKBP:FK506 complexes have been shown to inhibit calcineurin, a calcium and calmodulin dependent protein phosphatase which has been implicated as an important signaling enzyme in T-cell activation, providing a possible mechanism of immunosuppression by CsA and FK506. Immunophilins function as peptidyl prolyl cis-trans-isomerases (PPIase) whose activity is inhibited by their respective immunosuppressant compounds. As PPIase's, immunophilins accelerate folding of some proteins both in vivo and in vitro by catalyzing slow steps in the initial folding and rearrangement of proline containing proteins.CyP-40, a 40 kDa protein, shares significant homology with smaller CyPA (CyP-18) and FKBP59. CyP-40 exhibits the characteristic CsA binding and isomerase activity of CyP-18, though these activities appear to be less with CyP-40 than with Cyp-18. Like FKBP59, CyP-40 has been found in progesterone receptor complexes. CyP-40 is expressed at similar levels in many tissues. |
| Storage: | -20º C, Avoid Freeze/Thaw Cycles |
| Purity: | Whole antisera |
| Isotype: | IgG |
| Host Name: | Rabbit |
| Buffer: | PBS |
| Application Summary: | Western Blot 1:500 |
| Species Summary: | Hu, Rt, |
| Alternate Names: | anti-40 kDa peptidyl prolyl cis trans isomerase antibody, anti-40 kDa peptidyl prolyl cis trans isomerase D antibody, anti-Cyclophilin D antibody, anti-Cyclophilin related protein antibody, anti-Cyclophilin40 antibody, anti-CyclophilinD antibody, anti-CYP 40 antibody, anti-CYP40 antibody, anti-CYPD antibody, anti-MGC33096 antibody, anti-Peptidyl Prolyl Isomerase D antibody, anti-Peptidylprolyl isomerase D antibody, anti-PPIase antibody, anti-PPID antibody, anti-Rotamase antibody |
| Package Size: | 0.1 ml |
| Preservative: | 0.05% sodium azide |
| General References: | General / background references:Kieffer LJ et al. Cyclophilin-40, a protein with homology to the P59 component of the steroid receptor complex. Cloning of the cDNA and further characterization. J Biol Chem 268:12303-10 (1993). Kieffer LJ et al. Isolation and characterization of a 40-kDa cyclophilin-related protein. J Biol Chem 267:5503-7 (1992). |
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