| CatalogCode: | NB120-5812 |
| ProductName: | PKC theta Antibody |
| Product Description: | Rabbit Polyclonal anti-PKC theta [Ser695] |
| Clonality: | Polyclonal |
| Immunogen: | Synthetic phosphopeptide derived from a region of human PKC theta that contains serine 695. |
| Specificity: | This antibody does not cross-react with any other PKC isoforms tested. |
| CrossReactivity: | Cross-reacts with Human.Expected to cross-react with Mouse (75% identity with immunogen) due to sequence homology.Not yet tested in other species. |
| Packaging: | 0.05 ml Immunogen affinity purified Rabbit IgG. |
| Uses: | WB: Use at a concentration of 0.1 - 1.0 ug/ml. Detects a band of approximately 80 kDa. Not tested in other applications.Optimal dilutions/concentrations should be determined by the end user. |
| Control: | Jurkat cells treated with PMA, a phorbol ester. |
| Background: | Protein Kinase C theta (PKC theta) is an 80 kDa member of the novel group (nPKCs: sensitive to diacylglycerol, phosphatidylserine and phorbol esters) of the PKC family of serine/threonine kinases that are involved in a wide range of physiological processes including mitogenesis, cell survival and homeostasis. Transgenic mice over-expressing dominant negative PKC theta develop hyperinsulinimia. PKC theta is involved in JNK activation and also plays a specialized role in TCR mediated activation of T and B cells. The activation of PKC theta in T cells is associated with its recruitment to the membrane, and is mediated by PI3-kinase and Vav. PKC theta is a constitutively competent kinase and is auto-phosphorylated on serine 676 in the turn loop and serine 695 in the hydrophobic loop. The phosphorylation on serine 695 contributes to the catalytic activity of PKC theta. |
| Storage: | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze thaw cycles. |
| Purity: | Immunogen affinity purified |
| Isotype: | IgG |
| Host_Name: | Rabbit |
| Buffer: | phosphate buffered saline (without Mg2+ and Ca2+), pH 7.3 (+/- 0.1), 50% glycerol with 1.0 mg/mL BSA |
| ListPrice: | 285 |
| AppSummary: | WB |
| SpeciesSummary: | Hu |
| ALTnames: | anti-PKC 0 antibody, anti-PKC theta antibody, anti-PKC-0 antibody, anti-PKC0 antibody, anti-PRKCQ antibody, anti-Protein Kinase C theta antibody |
| ProteinTarget: | PKC theta phospho S695 |
| PackageSize: | 0.05 ml |
| GeneralRef: | General / background references:Dennehy KM et al. Mitogenic signals through CD28 activate the protein kinase Ctheta-NF-kappaB pathway in primary peripheral T cells. Int Immunol 15:655-63 (2003). Liu Y et al. Phosphorylation of the protein kinase C-theta activation loop and hydrophobic motif regulates its kinase activity, but only activation loop phosphorylation is critical to in vivo nuclear-factor-kappaB induction. Biochem J 361:255-65 (2002). Gao T & Newton AC The turn motif is a phosphorylation switch that regulates the binding of Hsp70 to protein kinase C. J Biol Chem 277:31585-92 (2002). Villalba M et al. Translocation of PKC[theta] in T cells is mediated by a nonconventional, PI3-K- and Vav-dependent pathway, but does not absolutely require phospholipase C. J Cell Biol 157:253-63 (2002). |
| Phosphorylated: | Ser695 |
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