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| Catalog Code: | NB120-5798 |
| Product Name: | PKC eta Phosphospecific Antibody |
| Product Description: | Rabbit Polyclonal anti-PKC eta [Thr655] |
| Clonality: | Polyclonal |
| ListPrice: | 325 |
| Concentration: | 0.35 mg/ml |
| Immunogen: | Synthetic phosphopeptide derived from a region of human PKC eta that contains threonine 655. |
| Specificity: | This antibody does not cross-react with any other PKC isoforms tested. |
| Cross Reactivity: | Cross-reacts with Human. Expected to cross-react with Mouse (100% identity with immunogen) and Rat (100% identity with immunogen) due to sequence homology. Not yet tested in other species. |
| Packaging: | 0.05 ml Immunogen affinity purified Rabbit antisera. |
| Uses: | WB: Use at a concentration of 0.35 - 1.0 ug/ml. Detects a band of approximately 80 kDa. |
| Background: | Protein Kinase C eta (PKC eta) is an 80 kDa member of the novel group (nPKCs: sensitive to diacylglycerol, phosphatidylserine and phorbol esters) of the PKC family of serine/threonine kinases that are involved in a wide range of physiological processes including mitogenesis, cell survival, transcriptional regulation and tumor promotion. PKC eta has been shown to be involved in the cell cycle as ectopic expression results in elevated expression of cyclins D and E, and its association with the cyclinE/cdk2/p21 complex inhibits cdk2 kinase activity, leading to G1 arrest. PKC eta is also involved in mediating FGF and PDGF ability to stimulate IL-6 production, and PKC eta modulation of ERK and JNK signaling pathways. The activation loop threonine (threonine 512 in PKC eta) of novel PKCs is phosphorylated by phosphoinositide dependent kinase-1 (PDK1) and is necessary for the autophosphorylation of threonine 655 in the carboxy-terminus of PKC eta, a step that may play a role in mediating PKC eta signaling events. |
| Storage: | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze thaw cycles. |
| Purity: | Immunogen affinity purified |
| Isotype: | IgG |
| Host Name: | Rabbit |
| Buffer: | PBS, 1mg/ml BSA. pH 7.3 |
| Application Summary: | Western Blot 0.35-1 ug/ml |
| Species Summary: | Hu, Mu, Rt, |
| Alternate Names: | anti-MGC5363 antibody, anti-PKCL antibody, anti-PRKCH antibody, anti-PRKCL antibody, anti-Protein kinase C eta antibody, anti-Protein kinase C eta type antibody |
| Package Size: | 0.05 ml |
| Preservative: | 0.05% Sodium Azide |
| Phosphorylated: | Thr655 |
| General References: | General / background references:Brandlin I et al. Protein kinase C (PKC)eta-mediated PKC mu activation modulates ERK and JNK signal pathways. J Biol Chem 277:6490-6 (2002). Thabard W et al. Protein kinase C delta and eta isoenzymes control the shedding of the interleukin 6 receptor alpha in myeloma cells. Biochem J 358:193-200 (2001). Kashiwagi M et al. PKCeta associates with cyclin E/cdk2/p21 complex, phosphorylates p21 and inhibits cdk2 kinase in keratinocytes. Oncogene 19:6334-41 (2000). Waldron RT et al. The pleckstrin homology domain of protein kinase D interacts preferentially with the eta isoform of protein kinase C. J Biol Chem 274:9224-30 (1999). |
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