| CatalogCode: | NB120-5797 |
| ProductName: | PKC gamma Antibody |
| Product Description: | Rabbit Polyclonal anti-PKC gamma [Thr674] |
| Clonality: | Polyclonal |
| Immunogen: | Synthetic phosphopeptide derived from a region of human PKC gamma that contains threonine 674. |
| Specificity: | PKC alpha (69%) may cross-react in cells expressing a high level of this protein. The peptide competition data (see Figure) suggest that this antibody will cross-react with PKC alpha [pS657] (69% homologous), beta 1 [pS661] (54%) and PKC eta [pS674] (38%). |
| CrossReactivity: | Cross-reacts with Human.Expected to cross-react with Mouse (100% identity with immunogen) and Rat (100% identity with immunogen) due to sequence homology.Not yet tested in other species. |
| Packaging: | 0.05 ml Immunogen affinity purified Rabbit IgG. |
| Uses: | WB: Use at a concentration of 0.35 - 1.0 ug/ml. Detects a band of approximately 80 kDa. Not tested in other applications.Optimal dilutions/concentrations should be determined by the end user. |
| Localization: | Cytoplasmic and Plasma membrane |
| Control: | HeLa cells treated with PMA, a phorbol ester. |
| Background: | Protein Kinase C gamma (PKC gamma) is an 80 kDa member of the conventional grou(cPKCs: sensitive to calcium, diacylglycerol, phosphotidylserine and phorbol esters) of the PKC family of serine/threonine kinases that are involved in a wide range of physiological processes including mitogenesis, cell survival and transcriptional regulation. PKC gamma plays a key role in neuronal signal transduction and in regulating intercellular communication. The activation loop threonine (threonine 514 in PKC gamma) of conventional PKCs is phosphorylated by phosphoinositide-dependent kinase-1 (PDK1), which is necessary for its autophosphorylation on threonine 655 in the turn loop, and threonine 674 in the hydrophobic loop of the carboxy terminus, a critical step in generating a catalytically mature enzyme. The phosphorylation of the hydrophobic loop in the carboxyl terminus of PKCs is believed to be a key determinant in regulating PKC interaction with PDK1. |
| Storage: | Store at −20oC. We recommend a brief centrifugation before opening to settle vial contents. Then, apportion into working aliquots and store at -20oC. For shipment and short-term storage (up to one week), 2-8oC is sufficient. |
| Purity: | Immunogen affinity purified |
| Isotype: | IgG |
| Host_Name: | Rabbit |
| Buffer: | phosphate buffered saline (without Mg2+ and Ca2+), pH 7.3 (+/- 0.1), 50% glycerol with 1.0 mg/mL BSA |
| ListPrice: | 285 |
| AppSummary: | WB |
| SpeciesSummary: | Hu |
| ALTnames: | anti-PKCC antibody, anti-PKCG antibody, anti-Protein kinase C gamma antibody, anti-Protein kinase C gamma polypeptide antibody, anti-SCA14 antibody |
| ProteinTarget: | PKC gamma phospho T674 |
| PackageSize: | 0.05 ml |
| GeneralRef: | General / background references:Nguyen TA et al. LEDGF activation of PKC gamma and gap junction disassembly in lens epithelial cells. Exp Eye Res 76:565-72 (2003). Aronowski J et al. Interplay between the gamma isoform of PKC and calcineurin in regulation of vulnerability to focal cerebral ischemia. J Cereb Blood Flow Metab 20:343-9 (2000). Dutil EM et al. Regulation of conventional protein kinase C isozymes by phosphoinositide-dependent kinase 1 (PDK-1). Curr Biol 8:1366-75 (1998). Toker A Signaling through protein kinase C. Front Biosci 3:D1134-47 (1998). |
| Phosphorylated: | Thr674 |
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