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| Catalog Code: | NB120-5796 |
| Product Name: | PKC gamma Phosphospecific Antibody |
| Product Description: | Rabbit Polyclonal anti-PKC gamma [Thr655] |
| Clonality: | Polyclonal |
| ListPrice: | 325 |
| Concentration: | 0.5 mg/ml |
| Immunogen: | Synthetic phosphopeptide derived from a region of human PKC gamma that contains threonine 655. |
| Specificity: | PKC alpha (69%) and beta 2 (62%) may cross-react in cells expressing high levels of these proteins. |
| Cross Reactivity: | Cross-reacts with Human.Expected to cross-react with Mouse (100% identity with immunogen) and Rat (100% identity with immunogen) due to sequence homology.Not yet tested in other species. |
| Packaging: | 0.05 ml Immunogen affinity purified Rabbit IgG. |
| Uses: | WB: Use at a concentration of 0.5 - 1.0 ug/ml. Detects a band of approximately 80 kDa. Not tested in other applications.Optimal dilutions/concentrations should be determined by the end user. |
| Localization: | Cytoplasmic |
| Background: | PKC gamma is an 80 kDa member of the conventional group (cPKCs: sensitive to calcium, diacylglycerol and phorbol esters) of the PKC family of serine/threonine family kinases that are involved in a wide range of physiological processes including mitogenesis, cell survival and transcriptional regulation. PKC gamma plays a key role in neuronal signal transduction and is translocated from the nucleus to the cytoplasm upon activation by phorbol ester, where in epithelial cells it has been implicated in regulating intracellular communication. The activation loop threonine (threonine 514 in PKC gamma) of conventional PKCs is phosphorylated by phosphoinositide-dependent kinase-1 (PDK1), which is necessary for their autophosphorylation, a critical step in the generation of a catalytically mature enzyme. Threonine 655 is an autophosphorylation site in the carboxy-terminus of PKC gamma. |
| Storage: | Store at −20oC. We recommend a brief centrifugation before opening to settle vial contents. Then, apportion into working aliquots and store at -20oC. For shipment and short-term storage (up to one week), 2-8oC is sufficient. |
| Purity: | Immunogen affinity purified |
| Isotype: | IgG |
| Host Name: | Rabbit |
| Buffer: | phosphate buffered saline (without Mg2+ and Ca2+), pH 7.3 (+/- 0.1), 50% glycerol with 1.0 mg/mL BSA |
| Application Summary: | Western Blot |
| Species Summary: | Hu |
| Alternate Names: | anti-PKCC antibody, anti-PKCG antibody, anti-Protein kinase C gamma antibody, anti-Protein kinase C gamma polypeptide antibody, anti-SCA14 antibody |
| Package Size: | 0.05 ml |
| Preservative: | 0.05% sodium azide |
| Phosphorylated: | Thr655 |
| General References: | General / background references:Wagner LM et al. Effect of protein kinase Cgamma on gap junction disassembly in lens epithelial cells and retinal cells in culture. Mol Vis 8:59-66 (2002). Dutil EM et al. Regulation of conventional protein kinase C isozymes by phosphoinositide-dependent kinase 1 (PDK-1). Curr Biol 8:1366-75 (1998). Machide M et al. Selective activation of phospholipase C gamma1 and distinct protein kinase C subspecies in intracellular signaling by hepatocyte growth factor/scatter factor in primary cultured rat neocortical cells. J Neurochem 71:592-602 (1998). Battaini F et al. Protein kinase C activity, translocation, and conventional isoforms in aging rat brain. Neurobiol Aging 16:137-48 (1995). |
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