| CatalogCode: | NB120-5780 |
| ProductName: | PKC alpha Antibody |
| Product Description: | Rabbit Polyclonal anti-PKC alpha [Thr638] |
| Clonality: | Polyclonal |
| Immunogen: | Synthetic phosphopeptide derived from the region of human PKC alpha that contains threonine 638 |
| Specificity: | PKC beta 2 (77% homologous) and gamma (69% homologous) may cross-react in cells expressing high levels of these proteins. However, our data (see image) only demonstrates cross-reactivity with the homologous PKC beta 2 sequence. |
| CrossReactivity: | Cross-reacts with Human and Mouse.Expected to cross-react with Rat (100% identity with immunogen) due to sequence homology.Not yet tested in other species. |
| Packaging: | 0.05 ml Immunogen affinity purified Rabbit IgG. |
| Uses: | WB: Use at a concentration of 0.35 - 1.0 ug/ml. Detects a band of approximately 80 kDa. Not tested in other applications.Optimal dilutions/concentrations should be determined by the end user. |
| Localization: | Cytoplasmic and Nuclear |
| Control: | U937 cells and NIH3T3 cells. |
| Background: | Protein Kinase c alpha (PKC alpha) is an 77 kDa member of the conventional group (cPKCs: sensitive to calcium, diacylglycerol, phosphatidylserine and phorbol esters) of the PKC family of serine/ threonine kinases that are involved in a wide range of physiological processes including mitogenesis, cell survival and transcriptional regulation. PKC alpha is an ubiquitously expressed PKC isozyme that has been implicated in the regulation of a broad range of cellular functions including proliferation, differentiation, development, migration, cell-cell adhesion, cell extracellular matrix adhesion, and solute transport. The activation loop threonine (threonine 497 in PKC alpha) of conventional PKCs is phosphorylated by phosphoinositide-dependent kinase-1 (PDK1). This phosphorylation is necessary for the autophosphorylation of threonine 638 in the carboxy-terminus of PKC alpha, a step that is critical for regulating the rate of PKC alpha dephosphorylation and inactivation. |
| Storage: | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze thaw cycles. |
| Purity: | Immunogen affinity purified |
| Isotype: | IgG |
| Host_Name: | Rabbit |
| Buffer: | phosphate buffered saline (without Mg2+ and Ca2+), pH 7.3 (+/- 0.1), 50% glycerol with 1.0 mg/mL BSA |
| ListPrice: | 285 |
| AppSummary: | WB |
| SpeciesSummary: | Hu, Mu |
| ALTnames: | anti-Protein Kinase C antibody |
| ProteinTarget: | PKC alpha phospho T638 |
| PackageSize: | 0.05 ml |
| GeneralRef: | General / background references:Newton AC Regulation of the ABC kinases by phosphorylation: protein kinase C as a paradigm. Biochem J 370:361-71 (2003). da Rocha AB et al. Targeting protein kinase C: new therapeutic opportunities against high-grade malignant gliomas? Oncologist 7:17-33 (2002). Bornancin F & Parker PJ Phosphorylation of threonine 638 critically controls the dephosphorylation and inactivation of protein kinase Calpha. Curr Biol 6:1114-23 (1996). |
| Phosphorylated: | Thr638 |