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| Catalog Code: | NB120-5777 |
| Product Name: | RSK1 p90 Phosphospecific Antibody |
| Product Description: | Rabbit Polyclonal anti-RSK1 p90 [Ser380] |
| Clonality: | Polyclonal |
| ListPrice: | 325 |
| Concentration: | 1.0 mg/ml |
| Immunogen: | Synthetic phosphopeptide derived from a region of human RSK1 that contains serine 380. |
| Specificity: | Cross-reaction with RSK2 (85% homologous) is expected based on peptide competition by the analogous sequence from RSK2. Cross-reaction with RSK3 (77% homologous) and RSK4 (85% homologous) is possible but has not been tested. |
| Cross Reactivity: | Cross-reacts with Human and Mouse.Expected to cross-react with Rat (100% identity with immunogen) due to sequence homology. Not yet tested in other species. |
| Packaging: | 0.05 ml Immunogen affinity purified Rabbit antisera. |
| Uses: | WB: Use at a dilution of 1/1000. Detects a band of approximately 88 kDa. |
| Background: | RSK1 (also called MAPK Activated Protein Kinase-1a; MAPKAP-K1a) is a member of the broadly expressed p90 Ribosomal S6 Kinase (RSK) family of serine/threonine kinases that also includes RSK2 (MAPKAP-K1b), RSK3 (MAPKAP-K1c), and RSK4. RSK proteins are substrates for and downstream transducers of MAPK signaling proteins, primarily ERK1&2. RSK proteins possess two separate kinase domains, the C-terminal kinase domain and the N-terminal kinase domain, separated by a linker region containing a hydrophobic motif. Activation of RSK1 is a multi-step process involving phosphorylation of multiple residues within the three domains, changes in RSK protein localization, and modulation of RSK complex formation with ERK1&2 and/or 14-3-3 beta proteins. RSK is activated by many stimuli including growth factors, phorbol esters, cAMP, heat shock, and irradiation. ERK 1&2 phosphorylates threonine 573 of the C-terminal kinase domain, thereby stimulating this kinase activity and leading to phosphorylation of serine 380 within the linker region. Phosphoserine 380 is part of a docking site for PDK1, a constitutively active cytoplasmic kinase that phosphorylates serine 221 and thereby activates the N-terminal kinase domain to phosphorylate various substrates that include transporters, transcription factors, and transcription co-activators. |
| Storage: | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze thaw cycles. |
| Purity: | Immunogen affinity purified |
| Isotype: | IgG |
| Host Name: | Rabbit |
| Buffer: | 50% Glycerol, PBS, 1mg/ml BSA. pH 7.3 |
| Application Summary: | Western Blot 1:1000 |
| Species Summary: | Hu, Mu, Rt, |
| Alternate Names: | anti-HU 1 antibody, anti-MAPKAPK1A antibody, anti-Ribosomal protein S6 kinase 90kD 1 antibody, anti-Ribosomal protein S6 kinase 90kD polypeptide 1 antibody, anti-RSK 1 antibody, anti-RSK antibody, anti-RSK-1 antibody |
| Package Size: | 0.05 ml |
| Preservative: | Sodium Azide |
| Phosphorylated: | Ser380 |
| General References: | General / background references:Roux PP et al. Phosphorylation of p90 ribosomal S6 kinase (RSK) regulates extracellular signal-regulated kinase docking and RSK activity. Mol Cell Biol 23:4796-804 (2003). Kim KW et al. Extracellular signal-regulated kinase/90-KDA ribosomal S6 kinase/nuclear factor-kappa B pathway mediates phorbol 12-myristate 13-acetate-induced megakaryocytic differentiation of K562 cells. J Biol Chem 276:13186-91 (2001). Richards SA et al. Characterization of regulatory events associated with membrane targeting of p90 ribosomal S6 kinase 1. Mol Cell Biol 21:7470-80 (2001). Zhang Y et al. UVA induces Ser381 phosphorylation of p90RSK/MAPKAP-K1 via ERK and JNK pathways. J Biol Chem 276:14572-80 (2001). |
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