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| Catalog Code: | NB120-5714 |
| Product Name: | Bax Antibody |
| Product Description: | Mouse Monoclonal anti-Bax (6A7) |
| Clone: | 6A7 |
| Clonality: | Monoclonal |
| ListPrice: | 295 |
| Concentration: | 2.0 mg/ml |
| Immunogen: | Synthetic peptide conjugated to KLH, corresponding to amino acids 12-24 of Human Bax. |
| Cross Reactivity: | Cross-reacts with Human, Mouse and Rat.Not yet tested in other species. |
| Packaging: | 0.1 ml Affinity purified Mouse ascites. |
| Uses: | Immunoprecipitation and Western Blot: In WB detects a band of approximately 20-22 kDa. Optimal dilutions/concentrations should be determined by the end user. |
| Localization: | Cell Membrane (alpha isoform) and Cytoplasmic |
| Background: | Apoptosis is an active process of cell death that controls cell numbers in a variety of tissues during embryonic development and throughout adult life. The prototypic regulator of mammalian cell death is the protooncogene bcl-2. In both normal and neoplastic tissues and in experimental situations, expression or overexpression of the bcl-2 gene appears to protect cells from death, by preventing or delaying apoptosis. Also, other genes seem to be important in controlling cell death. Candidates include bcl-x, bad, bak and bax, which have a significant homology to bcl-2. The bcl-x gene encodes two proteins: Bcl-xL (a 241 a.a. protein), which like Bcl-2, promotes cell survival, and Bcl-xS (deleted in 63 a.a.) , a splice variant of Bcl-xL that antagonizes Bcl-2 function. On the other hand, Bad and Bax enhance apoptosis and inhibit the protective functions of Bcl-xL (and to a lesser extent of Bcl-2) and Bcl-2, respectively. Bcl-2, Bcl-xL and Bax, each contain a stretch of hydrophobic amino acids, approx. 20 residues in length, at their C-termini. There is little amino acid sequence conservation within these tails, but based on hydropathy plot analysis they are presumed to function in anchoring these proteins into organelle membranes. Bcl-2 (a 26 kDa protein) has been localized to the nuclear membrane, endoplasmic reticulum, and the outer mitochondrial membranes. Bcl-xL (27 kDa) has been localized to the outer membrane of mitochondria. Bax (21 kDa) is an integral organelle membrane protein, in particular in mitochondria. However, significant amounts of Bcl-xL and most of the Bax proteins are not membraneassociated and appear to be cytosolic, according to other reports. Bax is associated with organelles or bound to organelles by Bcl-2 or a soluble protein found in the cytosol. Formation of Bax homodimers promotes cell death, and this could be blocked by Bax heterodimerization with Bcl-2 or Bcl-xL. Although the relative ratio of Bax homodimers to heterodimers has been proposed to serve as a sensory switch to regulate cell death, this interaction is promoted by the presence of nonionic detergents, which stimulate Bax dimer formation. Other hypotheses propose the formation of channels in mitochondrial outer membranes, or the interaction of these members with the PTP pore to regulate the release of cytochrome c. Cytochrome c in turn activates caspase-3 to cause cell death. Antibodies reacting specifically with Bax protein are useful tools in the study of the unique subcellular localization of Bax, and of the intracellular redistribution of this protein upon induction of apoptosis. |
| Storage: | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze thaw cycles. |
| Purity: | Affinity purified |
| Isotype: | IgG1 |
| Host Name: | Mouse |
| Buffer: | Phosphate-buffered saline, pH 8. |
| Application Summary: | immunoprecipitation assay dependent, Western Blot 1 - 2 ug/ml |
| Species Summary: | Hu, Mu, Rt, |
| Alternate Names: | anti-apoptosis regulator BAX antibody, anti-Apoptosis regulator BAX cytoplasmic isoform beta antibody, anti-Apoptosis regulator BAX membrane isoform alpha antibody, anti-Bax isoform psi antibody, anti-BAX protein cytoplasmic isoform delta antibody, anti-Bax protein cytoplasmic isoform delta. antibody, anti-Bax protein cytoplasmic isoform gamma antibody, anti-Bax zeta antibody, anti-BCL2 associated X protein antibody |
| Package Size: | 0.1 ml |
| Preservative: | No Preservative |
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