| CatalogCode: | NB120-5676 |
| ProductName: | PKC theta Antibody |
| Product Description: | Rabbit Polyclonal anti-PKC theta [Ser676] |
| Clonality: | Polyclonal |
| Immunogen: | Synthetic phosphopeptide derived from a region of human PKC theta that contains serine 676. |
| Specificity: | This antibody did not cross-react with any other PKC isoforms tested. |
| CrossReactivity: | Cross-reacts with Human.Expected to cross-react with Mouse (93% identity with immunogen) due to sequence homology.Not yet tested in other species. |
| Packaging: | 0.05 ml Immunogen affinity purified Rabbit IgG. |
| Uses: | WB: Use at a concentration of 0.35 - 1 ug/ml. Detects a band of approximately 80 kDa. Not tested in other applications.Optimal dilutions/concentrations should be determined by the end user. |
| Localization: | Cytoplasmic |
| Control: | Jurkat cells treated with PMA, a phorbol ester. |
| Background: | Protein Kinase C theta (PKC theta) is an 80 kDa member of the novel group (nPKCs: sensitive to diacylglycerol, phosphatidylserine and phorbol esters) of the PKC family of serine/threonine kinases that are involved in a wide range of physiological processes including mitogenesis, cell survival and transcriptional regulation. PKC theta plays a critical role in JNK activation, suggesting a role in the signaling processes of many biological responses such as inflammation and immune response, and also can play a specialized role in TCR mediated activation of T and B cells. The activation of PKC theta in T cells has been shown be linked to its recruitment to the membrane, and is mediated by PI3 kinase and Vav. PKC theta is a constitutively competent kinase and is autophosphorylated on serine 676 in the turn loop and serine 695 in the hydrophobic loop. Phosphorylation of serine 676 has minimal influence on the kinase activity of PKC theta but is believed to negatively regulate other PKC theta functions such as PKC theta mediated activation of NF kappa B. |
| Storage: | Store at −20oC. We recommend a brief centrifugation before opening to settle vial contents. Then, apportion into working aliquots and store at -20oC. For shipment and short-term storage (up to one week), 2-8oC is sufficient. |
| Purity: | Immunogen affinity purified |
| Isotype: | IgG |
| Host_Name: | Rabbit |
| Buffer: | phosphate buffered saline (without Mg2+ and Ca2+), pH 7.3 (+/- 0.1), 50% glycerol with 1.0 mg/mL BSA |
| ListPrice: | 285 |
| AppSummary: | WB |
| SpeciesSummary: | Hu |
| ALTnames: | anti-PKC 0 antibody, anti-PKC theta antibody, anti-PKC-0 antibody, anti-PKC0 antibody, anti-PRKCQ antibody, anti-Protein Kinase C theta antibody |
| ProteinTarget: | PKC theta phospho S676 |
| PackageSize: | 0.05 ml |
| GeneralRef: | General / background references:Dennehy KM et al. Mitogenic signals through CD28 activate the protein kinase Ctheta-NF-kappaB pathway in primary peripheral T cells. Int Immunol 15:655-63 (2003). Liu Y et al. Phosphorylation of the protein kinase C-theta activation loop and hydrophobic motif regulates its kinase activity, but only activation loop phosphorylation is critical to in vivo nuclear-factor-kappaB induction. Biochem J 361:255-65 (2002). Gao T & Newton AC The turn motif is a phosphorylation switch that regulates the binding of Hsp70 to protein kinase C. J Biol Chem 277:31585-92 (2002). Villalba M et al. Translocation of PKC[theta] in T cells is mediated by a nonconventional, PI3-K- and Vav-dependent pathway, but does not absolutely require phospholipase C. J Cell Biol 157:253-63 (2002). |
| Phosphorylated: | Ser676 |
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