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| Catalog Code: | NB120-5663 |
| Product Name: | PDE6 Antibody |
| Product Description: | Pig Polyclonal anti-PDE6 |
| Clonality: | Polyclonal |
| ListPrice: | 295 |
| Concentration: | 1 mg/ml |
| Immunogen: | Synthetic peptide: HQYFG(K/R)KLSPENVAGAC, corresponding to amino acids 20-36 of Mouse and Cow PDE6 beta. |
| Cross Reactivity: | Cross-reacts with Mouse. Not yet tested in other species. |
| Packaging: | 0.1 mg Immunogen affinity purified Pig antisera. |
| Uses: | IF: Use at an assay dependent dilution.IHC-Fr: Use at an assay dependent dilution. WB: Use at a concentration of 2 ug/ml. Detects a band of approximately 90 kDa. Not tested in other applications.Optimal dilutions/concentrations should be determined by the end user. |
| Localization: | Plasma membrane |
| Background: | The second messengers cAMP and cGMP are key regulatory molecules that are involved in a wide variety of signal transduction pathways, such as insulin secretion, platelet aggregation, smooth muscle relaxation, olfaction, and vision. Levels of cAMP and cGMP are regulated by their rate of synthesis by nucleotide cyclases and by their rate of hydrolysis by cyclic nucleotide phosphodiesterases (PDEs). PDEs form a superfamily of enzymes that catalyze the conversion of 3-prime, 5-prime-cyclic nucleotides to the corresponding nucleoside 5-prime-monophosphates. While mammalian PDEs are divided into major families based on their substrate specificities, kinetic properties, allosteric regulators, inhibitor sensitivities, and amino acid sequences, each family and even members within a family display distinct tissue, cell, and subcellular expression patters. This suggests that individual PDE family members are involved in discrete signal transduction pathways. PDE6 is the effector enzyme in the G protein-mediated signal transduction cascade in the visual system. There are five different subunits consisting of rod and cone specific catalytic subunits: alpha? (Cone), alpha (Rod), and beta (Rod), the inhibitory subunit gamma, and subunit delta of unknown function (which likely interacts with many other proteins besides the PDE6 family). The catalytic core of the PDE6 system is comprised of alpha?/alpha? homodimers in the cone and alpha/beta heterodimers in the rod. The C-terminus of both the catalytic and inhibitory subunits is modified by methylation, myristyolation and prenylation which have been shown to be critical for proper complex assembly and membrane association. |
| Purity: | Immunogen affinity purified |
| Isotype: | IgG |
| Host Name: | Pig |
| Buffer: | Preservative: 0.05% Sodium AzideConstituents: PBS, 1mg/ml BSA |
| Application Summary: | immunofluorescence , Western Blot , Immunohistochemistry-Frozen |
| Species Summary: | Mu, |
| Package Size: | 0.1 mg |
| Preservative: | 0.05% sodium azide |
| General References: | He F <i>et al.</i> Multiple zinc binding sites in retinal rod cGMP phosphodiesterase, PDE6alpha beta. <i>J Biol Chem</i> <b>275</b>:20572-7 (2000). <PUBMED:10787404>Granovsky AE & Artemyev NO Identification of the gamma subunit-interacting residues on photoreceptor cGMP phosphodiesterase, PDE6alpha '. <i>J Biol Chem</i> <b>275</b>:41258-62 (2000). <PUBMED:11024033> |
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