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| Catalog Code: | NB120-5625 |
| Product Name: | CLOCK Antibody |
| Product Description: | Rabbit Polyclonal anti-CLOCK |
| Clonality: | Polyclonal |
| ListPrice: | 285 |
| Concentration: | 1 mg/ml |
| Immunogen: | Synthetic peptide: SLPETAADKSQDSGSDND, corresponding to amino acids 394-411 of Mouse CLOCK, this sequence is completely conserved between mouse and human CLOCK. |
| Specificity: | NB120-5625 detects CLOCK from cells transfected with the mouse CLOCK gene. |
| Cross Reactivity: | Cross-reacts with Mouse.Expected to cross-react with Human (100% identity with immunogen), Rat (100% identity with immunogen) due to sequence homology.Not yet tested in other species. |
| Packaging: | 0.2 ml Immunogen affinity purified Rabbit IgG. |
| Uses: | WB: Use at a dilution of 1/200. Detects a band of approximately 100 kDa (predicted molecular weight: 101.5 kDa). Not tested in other applications.Optimal dilutions/concentrations should be determined by the end user. |
| Localization: | Nuclear |
| Background: | Circadian rhythmicity is a basic property of phylogenetically diverse organisms which range from animals and plants, to fungi. Regulation of endogenous biological clocks is regulated at the genetic level by a protein-mediated, autoregulatory feed-back loop. In mammals, several genes that encode members of the basic helix-loop helix (bHLH) PAS (PER-ARNT-SIM) transcription factor family have been shown to play a significant role in regulating circadian oscillations. Transactivation of CLOCK-induced genes is mediated via an E box enhancer (CACGTG) found upstream of target genes. CLOCK-ARNT3 heterodimers bind to E box regulatory elements and stimulate gene transcription. CLOCK has been shown to transactivate the mammalian homolog of Drosophila per. PER, in concert with the product of the mammalian timeless gene (TIM), negatively regulates its own transcription by blocking the activity of the CLOCK-BMAL1 transactivation complex. |
| Storage: | -20º C, Avoid Freeze/Thaw Cycles |
| Purity: | Immunogen affinity purified |
| Isotype: | IgG |
| Host Name: | Rabbit |
| Buffer: | PBS |
| Application Summary: | Western Blot 1:200 |
| Species Summary: | Mu, |
| Alternate Names: | anti-Circadian locomoter output cycles kaput protein antibody, anti-Circadian Locomotor Output Cycles Kaput antibody, anti-Circadium Locomotor Output Cycles Kaput antibody, anti-Clock homolog antibody, anti-Clock protein antibody, anti-hCLOCK antibody, anti-KIAA0334 antibody |
| Package Size: | 0.2 ml |
| Preservative: | 0.05% Sodium Azide, 1mg/ml BSA |
| General References: | General / background references:Reppert SM A clockwork explosion! Neuron 21:1-4 (1998). Gekakis N et al. Role of the CLOCK protein in the mammalian circadian mechanism. Science 280:1564-9 (1998). Darlington TK et al. Closing the circadian loop: CLOCK-induced transcription of its own inhibitors per and tim. Science 280:1599-603 (1998). |
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