| CatalogCode: | NB120-5588 |
| ProductName: | nNOS Antibody |
| Product Description: | Rabbit Polyclonal anti-nNOS |
| Clonality: | Polyclonal |
| Immunogen: | Synthetic peptide: IQVDRDLDGKSHKA , corresponding to amino acids 237-250 of NOS (nNOS). |
| Specificity: | This antibody does not to detect iNOS or eNOS. |
| CrossReactivity: | Cross-reacts with Rat.Expected to cross-react with Human (100% identity with immunogen), Mouse (92% identity with immunogen) due to sequence homology.Not yet tested in other species. |
| Packaging: | 0.1 ml Immunogen affinity purified Rabbit IgG. |
| Uses: | WB: Use at a dilution of 1/1:100. Detects a band of approximately 155 kDa. Not tested in other applications.Optimal dilutions/concentrations should be determined by the end user. |
| Localization: | In skeletal muscle, it is localized beneath the sarcolemma of fast-twitch muscle fiber by associating with the dystrophin glycoprotein complex (by similarity). |
| Background: | Nitric oxide (NO) is an inorganic, gaseous free radical that carries a variety of messages between cells. Vasorelaxation, neurotransmission and cytotoxicity can all be potentiated through cellular response to NO. NO production is mediated by members of the nitric oxide synthase (NOS) family. NOS catalyzes the oxidization of L-arginine to produce L-citrulline and NO. Two constitutive isoforms, brain or neuronal NOS (b or nNOS, type I) & endothelial cell NOS (eNOS, type III), and one inducible isoform (iNOS, type II), have been cloned. All NOS isoforms contain calmodulin, nicotinamide adenine dinucleotide phosphate (NADPH), flavin adenine dinucleotide (FAD), and flavin mononucleotide (FMN) binding domains. bNOS and eNOS share approximately 50% sequence homology and their enzymatic activity depends on binding to the calcium/calmodulin complex. Increases in intracellular calcium lead to the production of low levels of NO over a short time. bNOS is found in neurons, peripheral nerve cells, macula densa, and pancreatic islet cells. Alternate splicing specifically regulates bNOS in striated muscle. The translated protein, bNOS mu, is 34 amino acid residues larger than bNOS from brain. |
| Storage: | Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles. |
| Purity: | Immunogen affinity purified |
| Isotype: | IgG |
| Host_Name: | Rabbit |
| Buffer: | PBS |
| ListPrice: | 285 |
| AppSummary: | WB |
| SpeciesSummary: | Rt |
| ALTnames: | anti-bNOS antibody, anti-Constitutive NOS antibody, anti-IHPS 1 antibody, anti-IHPS1 antibody, anti-N NOS antibody, anti-NC NOS antibody, anti-neuronal Nitric Oxide Synthase antibody, anti-Neuronal NOS antibody, anti-Nitric oxide synthase 1 (neuronal) antibody, anti-Nitric oxide synthase 1 antibody, anti-Nitric oxide synthase brain antibody, anti-nNOS antibody, anti-NO antibody, anti-NOS 1 antibody, anti-NOS antibody, anti-NOS type I antibody, anti-NOS1 antibody |
| ProteinTarget: | NOS (nNOS) |
| PackageSize: | 0.1 ml |
| GeneralRef: | General / background references:Silvagno F et al. Neuronal nitric-oxide synthase-mu, an alternatively spliced isoform expressed in differentiated skeletal muscle. J Biol Chem 271:11204-8 (1996). Stefanovic-Racic M et al. Nitric oxide and arthritis. Arthritis Rheum 36:1036-44 (1993). |
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