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| Catalog Code: | NB120-5586 |
| Product Name: | nNOS Antibody |
| Product Description: | Rabbit Polyclonal anti-nNOS |
| Clonality: | Polyclonal |
| ListPrice: | 285 |
| Concentration: | 1 mg/ml |
| Immunogen: | Synthetic peptide: CNRLRSESIAFIEESK , corresponding to amino acids 1409-1424 of Rat NOS (nNOS). |
| Specificity: | NB120-5586 detects nNOS from human samples. |
| Cross Reactivity: | Cross-reacts with Rat.Expected to cross-react with Human (100% identity with immunogen), Mouse (100% identity with immunogen), Rabbit (100% identity with immunogen), Xenopus laevis (100% identity with immunogen), Zebrafish (93% identity with immunogen) due to sequence homology.Not yet tested in other species. |
| Packaging: | 0.1 mg Immunogen affinity purified Rabbit IgG. |
| Uses: | WB: Use at a concentration of 2 ug/ml. Predicted molecular weight: 160 kDa. Not tested in other applications.Optimal dilutions/concentrations should be determined by the end user. |
| Localization: | In skeletal muscle, it is localized beneath the sarcolemma of fast-twitch muscle fiber by associating with the dystrophin glycoprotein complex (by similarity). |
| Background: | Nitric oxide (NO) is an inorganic, gaseous free radical that carries a variety of messages between cells. Vasorelaxation, neurotransmission and cytotoxicity can all be potentiated through cellular response to NO. NO production is mediated by members of the nitric oxide synthase (NOS) family. NOS catalyzes the oxidization of L-arginine to produce L-citrulline and NO. Two constitutive isoforms, brain or neuronal NOS (b or nNOS, type I) & endothelial cell NOS (eNOS, type III), and one inducible isoform (iNOS, type II), have been cloned. All NOS isoforms contain calmodulin, nicotinamide adenine dinucleotide phosphate (NADPH), flavin adenine dinucleotide (FAD), and flavin mononucleotide (FMN) binding domains. iNOS is found in a variety of cell types including macrophages, hepatocytes, synoviocytes, and smooth muscle cells. Cytokines such as interferon-gamma (IFN), tumor necrosis factor (TNF), interleukin-1 and -2, and lipopolysaccarides (LPS) cause an increase in iNOS mRNA, protein, and activity levels. Protein kinase C-stimulating agents exhibit the same effect on iNOS activity. After cytokine induction, iNOS exhibits a delayed activity response which is then followed by a significant increase in NO production over a long period of time. |
| Storage: | -20º C, Avoid Freeze/Thaw Cycles |
| Purity: | Immunogen affinity purified |
| Isotype: | IgG |
| Host Name: | Rabbit |
| Buffer: | PBS |
| Application Summary: | Western Blot 2 ug/ml |
| Species Summary: | Rt |
| Alternate Names: | anti-bNOS antibody, anti-Constitutive NOS antibody, anti-IHPS 1 antibody, anti-IHPS1 antibody, anti-N NOS antibody, anti-NC NOS antibody, anti-neuronal Nitric Oxide Synthase antibody, anti-Neuronal NOS antibody, anti-Nitric oxide synthase 1 (neuronal) antibody, anti-Nitric oxide synthase 1 antibody, anti-Nitric oxide synthase brain antibody, anti-nNOS antibody, anti-NO antibody, anti-NOS 1 antibody, anti-NOS antibody, anti-NOS type I antibody, anti-NOS1 antibody |
| Package Size: | 0.1 mg |
| Preservative: | 1mg/ml BSA, 0.05% Sodium Azide |
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