| CatalogCode: | NB120-5582 |
| ProductName: | Calpastatin Antibody |
| Product Description: | Mouse Monoclonal anti-Calpastatin (1F7E3D10) |
| Clone: | 1F7E3D10 |
| Clonality: | Monoclonal |
| Immunogen: | Purified bovine skeletal muscle 80 kDa subunit of m-Calpastatin. |
| Epitope: | This antibody recognizes an epitope between amino acid residues 543-673 (domain IV) of human calpastatin. |
| Specificity: | This antibody does not cross-react with calpains or calmodulin. |
| CrossReactivity: | Cross-reacts with Cow, Human, Pig and Rat.Not yet tested in other species. |
| Packaging: | 0.1 ml purified Mouse ascites. |
| Uses: | ICC: Use at a dilution of 1/200. WB: Use at a dilution of 1/5000. Detects bands of approximately 150, 125, 90 and 70 kDa (predicted molecular weight: 85 kDa) proteins from bovine skeletal muscle extract, which have been suggested to be proteins translated from different start sites of the calpastatin gene.Immunocytochemical staining of calpastatin in porcine LLC-PK1 cells with ab5582 results in diffuse cytoplasmic staining.Not tested in other applications.Optimal dilutions/concentrations should be determined by the end user. |
| Localization: | Cytoplasmic and Nuclear |
| Background: | The calpain system has been detected in every vertebrate tissue examined, and has been suggested to play a regulatory role in cellular protein metabolism. This regulatory role may have important implications in platelet aggregation and pathologies associated with altered calcium homeostasis and protein metabolism such as ischemic cell injury and degenerative diseases. Inhibitors of calpain have been shown to block dexamethasone and low-level irradiation induced apoptosis in thymocytes suggesting that calpain has a regulatory or mechanistic role in apoptotic cell death. Calpastatin, a specific endogenous inhibitor of calpain, has a predicted molecular weight of ~77 kDa and an internal repeat of four homologous domains which allow it to inhibit multiple calpain molecules simultaneously. It functions by binding to calpain when calpain binds calcium. When calpastatin is subjected to proteolytic digestion, fragments of calpastatin as small as 15 kDa still retain inhibitory activity. |
| Storage: | Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles. |
| Purity: | Ascites |
| Isotype: | IgG1 |
| Host_Name: | Mouse |
| ListPrice: | 285 |
| AppSummary: | WB, ICC, ICC |
| SpeciesSummary: | Bv, Hu, Po, Rt |
| ALTnames: | anti-Calpain inhibitor antibody, anti-CAST antibody, anti-Heart type calpastatin antibody, anti-MGC9402 antibody, anti-Sperm BS 17 component antibody |
| ProteinTarget: | Calpastatin |
| PackageSize: | 0.1 ml |
| GeneralRef: | General / background references:Boehm ML et al. Changes in the calpains and calpastatin during postmortem storage of bovine muscle. J Anim Sci 76:2415-34 (1998). Cong J et al. Effect of monoclonal antibodies specific for the 28-kDa subunit on catalytic properties of the calpains. J Biol Chem 268:25740-7 (1993). Lane RD et al. A comparison of the intracellular distribution of mu-calpain, m-calpain, and calpastatin in proliferating human A431 cells. Exp Cell Res 203:5-16 (1992). |