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| Catalog Code: | NB120-5577 |
| Product Name: | alpha B Crystallin Phosphospecific Antibody |
| Product Description: | Rabbit Polyclonal anti-alpha B Crystallin phospho S59 [Ser59] |
| Clonality: | Polyclonal |
| ListPrice: | 285 |
| Immunogen: | Synthetic peptide: FLRAPS(p)WIDTG, corresponding to amino acids 54-64 of Human Crystallin (alpha B). |
| Specificity: | This antibody does not detect the unphosphorylated form of the protein. |
| Cross Reactivity: | Cross-reacts with Cow, Human and Rat.Expected to cross-react with Hamster (91% identity with immunogen), Mouse (91% identity with immunogen), Rabbit (91% identity with immunogen) due to sequence homology.Not yet tested in other species. |
| Packaging: | 0.1 mg Immunogen affinity purified Rabbit IgG |
| Uses: | IF: Use at a concentration of 8 ug/ml. IHC-P: Use at a concentration of 8 ug/ml. WB: Use at a concentration of 0.5 ug/ml. Detects a band of approximately 20 kDa. Not tested in other applications.Optimal dilutions/concentrations should be determined by the end user. |
| Localization: | Cytoplasmic |
| Background: | Lens proteins consist almost entirely of crystallins (about 95%). Crystallins are also found vertebrate skeletal muscle tissue. In the lens, their structural function is to assist in maintaining the proper refractive index of the lens. The mammalian lens contains 3 major classes of crystallins: alpha, beta, and gamma. Alpha-crystallin is the largest of the crystallins and is composed of 2 primary gene products--alpha-A and alpha-B. There are at least 5 different proteins comprising the beta-crystallins. The gamma-crystallins are monomeric, but there are at least 5 gamma crystallins identified in bovine and rat lens. Alpha-Crystallin comprises 40% of total lens protein composition. In addition to maintaining proper refractive index, it also functions in a chaperone like manner by preventing the formation of aggregates possibly leading to cataract formation. It is believed that the phosphorylated states of the alpha-crystallin occur in response to cellular stress and may serve a structural control function and play a role in protein maintenance. Alpha-B crystallin has been linked to Alexander's disease where it accumulates in brain cells of those afflicted. |
| Storage: | -20º C, Avoid Freeze/Thaw Cycles |
| Purity: | Immunogen affinity purified |
| Isotype: | IgG |
| Host Name: | Rabbit |
| Buffer: | Constituents: PBS, 30mg/ml BSA |
| Application Summary: | immunofluorescence 8 ug/ml, Western Blot 0.5 ug/ml, Immunohistochemistry-Paraffin 8 ug/ml |
| Species Summary: | Bv, Hu, Rt, |
| Alternate Names: | anti-Alpha crystallin B chain antibody, anti-AACRYA antibody, anti-Alpha crystallin B chain antibody, anti-Alpha(B) crystallin antibody, anti-CRYA2 antibody, anti-CRYAB antibody, anti-Crystallin alpha B antibody, anti-Crystallin alpha polypeptide 2 antibody, anti-CTPP 2 antibody, anti-CTPP2 antibody, anti-Heat shock 20 kD like protein antibody, anti-Heat shock protein beta 5 antibody, anti-HSPB5 antibody, anti-NY REN 27 antigen antibody, anti-Renal carcinoma antigen NY REN 27 antibody, anti-Rosenthal fiber component antibody |
| Package Size: | 0.1 mg |
| Preservative: | 0.05% Sodium Azide |
| Phosphorylated: | Ser59 |
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more information: | company product webpage |