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| Catalog Code: | NB120-5503 |
| Product Name: | Triadin Antibody |
| Product Description: | Mouse Monoclonal anti-Triadin (IIG12) |
| Clone: | IIG12 |
| Clonality: | Monoclonal |
| ListPrice: | 285 |
| Immunogen: | Rabbit skeletal muscle triads. |
| Specificity: | This antibody does not detect triadin from cardiac tissues. |
| Cross Reactivity: | Cross-reacts with Mouse and Rabbit.Not yet tested in other species. |
| Packaging: | 0.1 ml purified Mouse ascites. |
| Uses: | IF: Use at a dilution of 1/1000. WB: Use at a dilution of 1/500. Detects a band of approximately 95 kDa representing triadin in rabbit skeletal muscle extracts. Immunofluorescence staining of triadin in rabbit skeletal muscle with ab5503 results in its co-localization with ryanodine receptor, which is consistent with the association of ryanodine receptor and triadin in triads.Not tested in other applications.Optimal dilutions/concentrations should be determined by the end user. |
| Background: | The junction between the transverse tubules (T-tubules) and the sarcoplasmic reticulum (SR) of skeletal muscle is called the triad. At the triad, dihydropyridine receptors (DHPR?s) of the T-tubule serve as voltage sensors in excitation-contraction coupling, while ryanodine receptors (RyR?s), the calcium release channels, exist in the membrane of the terminal cisternae of the SR. It is thought that during slow phase depolarization of the T-tubule, a third protein, triadin (MW 95 kDa) transmits electrochemical signals to the SR through direct interaction with both DHPR?s and RyR?s. Though its exact role in this signaling process is unclear, triadin has been shown to co-localize with both DHPR and RYR at the junctional face of the terminal cisternae. |
| Storage: | -20º C, Avoid Freeze/Thaw Cycles |
| Purity: | Ascites |
| Isotype: | IgG1 |
| Host Name: | Mouse |
| Buffer: | PBS |
| Application Summary: | immunohistochemistry 1:500, immunofluorescence , Western Blot 1:1,000 |
| Species Summary: | Mu, Rb, |
| Alternate Names: | anti-dJ166D18.1 antibody, anti-DKFZp779I2253 antibody, anti-MGC88285 antibody, anti-OTTHUMP00000040354 antibody, anti-TDN antibody, anti-TRDN antibody, anti-TRISK 51 antibody, anti-TRISK antibody, anti-TRISK51 antibody |
| Package Size: | 0.1 ml |
| Preservative: | 0.05% Sodium Azide |
| General References: | General / background references:Campbell KP et al. Identification and characterization of the high affinity [3H]ryanodine receptor of the junctional sarcoplasmic reticulum Ca2+ release channel. J Biol Chem 262:6460-3 (1987). Leung AT et al. Structural characterization of the 1,4-dihydropyridine receptor of the voltage-dependent Ca2+ channel from rabbit skeletal muscle. Evidence for two distinct high molecular weight subunits. J Biol Chem 262:7943-6 (1987). Imagawa T et al. Phosphorylation of the 1,4-dihydropyridine receptor of the voltage-dependent Ca2+ channel by an intrinsic protein kinase in isolated triads from rabbit skeletal muscle. J Biol Chem 262:8333-9 (1987). |
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