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| Catalog Code: | NB120-5479 |
| Product Name: | Hsp60 Antibody |
| Product Description: | Mouse Monoclonal anti-Hsp60 (2E1/53) |
| Clone: | 2E1/53 |
| Clonality: | Monoclonal |
| ListPrice: | 285 |
| Immunogen: | Human placental Hsp 60. |
| Epitope: | Epitope mapping studies using human Hsp 60 deletion mutants suggest that this antibody binds between amino acids 211-288. |
| Specificity: | Detects Hsp 60 from human cells, tissues and recombinant protein preparations. This antibody displays no other protein or species cross-reactivity. |
| Cross Reactivity: | Cross-reacts with Human.Not yet tested in other species. |
| Packaging: | 0.1 mg IgM purified Mouse IgM. |
| Uses: | ELISA: Use at an assay dependent dilution. IP: Use at an assay dependent dilution. WB: Use at a concentration of 10 ug/ml. Detects a band of approximately 58 kDa. Not tested in other applications.Optimal dilutions/concentrations should be determined by the end user. |
| Localization: | Mitochondrial/chloroplast |
| Background: | Heat shock proteins (HSP) are expressed in response to various biological stresses, including high temperatures. There are several major families of HSPs including HSP70, HSP90 and HSP100. HSP60, an ~60 kDa protein, is the human homologue of HSP65 (a major antigenic mycobacterial protein) and E. coli GroEL. HSP60, also referred to as chaperonin-60, P1 & mitonin, has been classified as a member of a family of proteins termed chaperonins which act to recognize and stabilize polypeptide intermediates during folding, assembly and disassembly. HSP60 is a constitutively expressed mitochondrial protein that shares many characteristics with HSP70 including high abundance, induction by environmental stress, ATPase activity and a critical role in protein assembly and folding. However, in contrast with HSP70, HSP60 functions as a 14 subunit oligomer which is thought to interact with only 1 or 2 target polypeptides at one time. |
| Storage: | -20º C, Avoid Freeze/Thaw Cycles |
| Purity: | IgM purified |
| Isotype: | IgM |
| Host Name: | Mouse |
| Buffer: | Reconstitute with PBS. |
| Application Summary: | ELISA , immunoprecipitation , Western Blot 10 µg/ml |
| Species Summary: | Hu, |
| Alternate Names: | anti-60 kDa chaperonin antibody, anti-60 kDa heat shock protein mitochondrial antibody, anti-Chaperonin antibody, anti-CPN 60 antibody, anti-CPN60 antibody, anti-GROEL antibody, anti-GroEL Homolog antibody, anti-Heat shock 60kD protein 1 chaperonin antibody, anti-Heat shock 60kDa protein 1 antibody, anti-Heat Shock Protein 60 antibody, anti-Heat shock protein 65 antibody, anti-Hsp 60 antibody, anti-HSP 65 antibody, anti-HSP65 antibody, anti-HSPD 1 antibody, anti-HSPD1 antibody, anti-HuCHA60 antibody, anti-Mitochondrial matrix protein P1 antibody, anti-P60 lymphocyte protein antibody, anti-Short heat shock protein 60 Hsp60s1 antibody, anti-Spastic paraplegia 13 antibody, anti-SPG 13 antibody, anti-SPG13 antibody |
| Package Size: | 0.1 mg |
| Preservative: | 1.0 mg/ml BSA and 0.05% sodium azide |
| General References: | General / background references:Rook G & McCulloch J HLA-DR4, mycobacteria, heat-shock proteins, and rheumatoid arthritis. Arthritis Rheum 35:1409-12 (1992). Sharif M et al. The development of monoclonal antibodies to the human mitochondrial 60-kd heat-shock protein, and their use in studying the expression of the protein in rheumatoid arthritis. Arthritis Rheum 35:1427-33 (1992). Langer T et al. Chaperonin-mediated protein folding: GroES binds to one end of the GroEL cylinder, which accommodates the protein substrate within its central cavity. EMBO J 11:4757-65 (1992). |
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